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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,451 results for: Ott

Showing: 6001 - 6020 of 8451 results

  1. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

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  6. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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  8. pOPINO_C5

    Plasmid
    #171925
    Purpose
    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C5
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171925-plasmid-map-sequence-id-338635

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  9. pOPINO_C1

    Plasmid
    #171924
    Purpose
    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171924-plasmid-map-sequence-id-338631

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  13. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

    Plasmid
    #122986
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(micro)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains R73Q "micro" mutation, Bo…
    Promoter
    human synapsin
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122986-plasmid-map-sequence-id-238430

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  14. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid
    #75123
    Purpose
    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag
    Depositor
    Christopher Vakoc
    Article
    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/
    Insert
    BRD9 (BRD9 Human)
    Use
    Retroviral
    Tags
    3x FLAG-tag
    Expression
    Mutation
    bromodomain replaced with the first bromodomain o…
    Promoter
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pOPINO_F2

    Plasmid
    #171926
    Purpose
    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_F2
    Use
    Tags
    His6
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171926-plasmid-map-sequence-id-338638

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Showing: 6001 - 6020 of 8451 results