We narrowed to 20,115 results for: IRE;

Showing: 9001 - 9030 of 20115 results

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-AP1/gamma1

Plasmid

#218801

Purpose

Mammalian expression of mouse Ap1 g1

Depositor

Marvin Bentley

Article

Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

Insert

Ap1 g1 (Ap1g1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSicoR human Drosha2

Plasmid

#14767

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pSuper mouse DGCR8-1

Plasmid

#14772

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pCPP5108

Plasmid

#128726

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-2

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5107

Plasmid

#128725

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAA1-1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

Plasmid

#22930

Purpose

Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1-deltaPH (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Mutation

Includes 127-1205 aa. Deleted amino-terminal PH d…

Promoter

CMV

Available Since

Feb. 3, 2010

Availability

Academic Institutions and Nonprofits only
pET14b PHAS-I

Plasmid

#15679

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I (Eif4ebp1 Rat)

Tags

6xHis

Expression

Bacterial

Mutation

Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
FAM19A2-V5

Plasmid

#184338

Purpose

Expression of V5-tagged mouse FAM19A2

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

FAM19A2 (Tafa2 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAGH42

Plasmid

#107249

Purpose

Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp

Use

Suicide vector for destined for double homologous…

Tags

mGFPmut3

Expression

Bacterial

Promoter

native promoter of sll0617

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mir124a

Plasmid

#61539

Purpose

Expresses mir124a in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

mir124a (MIR124-1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
BPK1179

Plasmid

#179296

Purpose

Expresses dCas9 fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-DmrA(x4)

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3626

Plasmid

#179630

Purpose

Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

RFP

Expression

Worm

Promoter

Pacr-2(s)

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EAAARx8-CaaX

Plasmid

#155224

Purpose

MPAct with increased search radius

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET-DUET-GST-eIF4G1-His6, eIF4E

Plasmid

#37232

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Inserts

TIF4631 (TIF4631 Budding Yeast)
CDC33 (CDC33 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCPP5537

Plasmid

#128730

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

HopAO1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL1-~100

Plasmid

#27176

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

MKL1 (MRTFA Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

Deletion of 100 aa from ATG

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCPP5099

Plasmid

#128714

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopK1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5328

Plasmid

#128721

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopU1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5306

Plasmid

#128712

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopH1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5571

Plasmid

#128713

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopI1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5539

Plasmid

#128728

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAF1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5618

Plasmid

#128709

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopE1

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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