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pYY166 pcDNA3.4-His-MBP-hAID

Plasmid

#231942

Purpose

Expression of His-MBP-AID fusion protein in mammalian cells

Depositor

Fei-Long Meng

Article

Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

Insert

AID (AICDA Human)

Tags

His-MBP

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAd-M3cherry

Plasmid

#61041

Purpose

This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

Depositor

Qiao Zhou

Article

Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

Inserts

Ngn3 (Neurog3 Mouse)
pdx1 (Pdx1 Mouse)
Mafa (Mafa Mouse)
monomeric cherry

Use

Adenoviral

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_WT

Plasmid

#81778

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

D40G

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R346C

Plasmid

#164213

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R346C

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Mutation

R235Q

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_FURIN_WT

Plasmid

#82122

Purpose

Gateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pcrDNA3.1-Chimera

Plasmid

#100891

Purpose

Mammalian expression of DNAJB1(exon1)-PRKACA(exon2-Cterminus) chimeric protein from fibrolamellar hepatocellular carcinoma

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Major Fibrolamellar hepatocellular carcinoma Chimera (DNAJB1, PRKACA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-Mettl3-dsRed-IRES-puro

Plasmid

#61516

Purpose

Constitutive expression of murine wild-type Mettl3

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Promoter

CAGG

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR1-P2A-mCherry

Plasmid

#203856

Purpose

Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR1 (EGR1 Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-HCN1 ion channel [N70/28R-1]

Plasmid

#206718

Purpose

Mammalian Expression Plasmid of anti-HCN1 ion channel (Rat). Derived from hybridoma N70/28-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN1 ion channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Hcn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-V842I

Plasmid

#116359

Purpose

Lentiviral expression of ERBB2 V842I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

V842I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel [K89/34R-2b]

Plasmid

#199404

Purpose

Mammalian expression plasmid of Anti-Kv2.1 K+ channel [K89/34R-2b] (Rat). Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kv2.1 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-D769H

Plasmid

#116327

Purpose

Lentiviral expression of ERBB2 D769H

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

D769H

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kir6.1 K+ channel [N366/60R]

Plasmid

#188206

Purpose

Mammalian Expression Plasmid of anti-Kir6.1 K+ channel (Rat). Derived from hybridoma N366/60

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kir6.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnj8 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

Plasmid

#192202

Purpose

Retro-HER2CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TASK1 K+ channel [N374/48R]

Plasmid

#188160

Purpose

Mammalian Expression Plasmid of anti-TASK1 K+ channel (Rat). Derived from hybridoma N374/48

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TASK1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnk3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kir2.1 K+ channel [N112B/22R]

Plasmid

#220400

Purpose

Mammalian Expression Plasmid of anti-Kir2.1 K+ channel (Mouse) IgG2a R-mAb. Derived from hybridoma N112B/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Kir2.1 K+ channel (Mus musculus) recombinant mouse monoclonal antibody. (Kcnj2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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