We narrowed to 15,180 results for: nol

1. Fgfr2-T2A-H2B-Halo donor

   Plasmid

   #113117

   Purpose

   Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Fgfr2 donor region including homology arms and T2A-H2B-Halo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ210

   Plasmid

   #109329

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gata3-T2A-H2B-eGFP donor

   Plasmid

   #113119

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata3 donor region including homology arms and T2A-H2B-eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAP003l2

   Plasmid

   #209748

   Purpose

   RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

   Depositor

   Ahmed Badran

   Article

   Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

   Insert

   NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB6371

   Plasmid

   #106153

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG1280 NLS-mCherry-FIREtag-NES

   Plasmid

   #205172

   Purpose

   expresses NLS-mCherry-FIREtag-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   NLS-mCherry-FIREtag-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS002

   Plasmid

   #194013

   Purpose

   This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

   Depositor

   Timothy Whitehead

   Article

   Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

   Insert

   C16 Ex3 XTR f1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABE8r-R153del

   Plasmid

   #208296

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-R153del-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCBB-ctH6-eGFP

   Plasmid

   #32562

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   6 His tag with Thrombin site

   Expression

   Mutation

   Promoter

   Consitutive lac

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-dCas9-PBE

    Plasmid

    #98159

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARK2aa

    Plasmid

    #81013

    Purpose

    E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-aphA
    and PMD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Fragment 1 Null

    Plasmid

    #198729

    Purpose

    Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    gRNA expression cassette in pENTR4
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL2620 (pTniQ_Vch)

    Plasmid

    #200892

    Purpose

    Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTniQ
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PD2529-CAG-Flag-mouse TGFb2

    Plasmid

    #180261

    Purpose

    Mouse full length TGFβ2 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    TGFβ2 (Tgfb2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HPRT

    Plasmid

    #22884

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Tags

    Expression

    Mutation

    PGK, NeoR, and BGH polyA are between the two geno…

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC12

    Plasmid

    #104786

    Purpose

    pNB184-Cas9 entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Use

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCellFree_G03 WAVE 2

    Plasmid

    #67098

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WAVE 2 (WASF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC10

    Plasmid

    #104784

    Purpose

    pAH595-gRNA 2xplex entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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