We narrowed to 10,611 results for: t7

1. MSX2-pET

   Plasmid

   #21025

   Depositor

   Corey Largman

   Article

   Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

   Insert

   MSX2 (Msx2 Mouse)
   

   Tags

   HIS and T7

   Expression

   Bacterial

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-VinculinI997A_TurboID_Venus

   Plasmid

   #200274

   Purpose

   Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

   Depositor

   Brenton Hoffman

   Article

   Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

   Insert

   VinculinI997A_TurboID_Venus
   

   Expression

   Mammalian

   Mutation

   change isoleucine to alanine at 997 of vinculin

   Promoter

   T7

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-BirA-CPDSalI

   Plasmid

   #38258

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   birA (birA E. coli)
   

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pGEX4T1-BirA

   Promoter

   T7

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. Pet15b-Internalization Control

   Plasmid

   #186553

   Purpose

   Negative Control mediating endosomal localization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-EGFP
   

   Tags

   8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ShadowG-SUMO1

   Plasmid

   #175239

   Purpose

   Bacterial expression and purification, conjugatable SUMO for SUMOylation

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SUMO1 (SUMO1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC-SmBiT

   Plasmid

   #207632

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-SmBiT
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDNA3-Cerulean-FHA1-NES

   Plasmid

   #138202

   Purpose

   Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   Cerulean-FHA1-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Spy0469

   Plasmid

   #159996

   Purpose

   Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   Spy0469 (24-389)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT138b-pro1

   Plasmid

   #138513

   Purpose

   P3h

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pJM126

    Plasmid

    #49339

    Purpose

    bacterial expression of RFA1, RFA2, and RFA3

    Depositor

    Steven Brill

    Article

    He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

    Inserts

    RFA3 (RFA3 Budding Yeast)
    RFA2 (RFA2 Budding Yeast)
    RFA1 (RFA1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pHis8-4b-SaDAH

    Plasmid

    #140128

    Purpose

    Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Sinomenium acutum
    

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTU425

    Plasmid

    #178984

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT H585A C627A
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-17b_Unc104-KIF5B-HALO

    Plasmid

    #169996

    Purpose

    Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

    Depositor

    Ron Vale

    Article

    Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

    Insert

    Kinesin-like protein Unc-104 fused with KIF5B
    

    Tags

    HALO-tag, 6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. M26I DJ-1 pET15b

    Plasmid

    #60677

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 RUVBL1

    Plasmid

    #67091

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G06_ZIKV NS3

    Plasmid

    #145355

    Purpose

    Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS3
    

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-Rab10-FLAG

    Plasmid

    #135559

    Purpose

    Express mouse Rab10 in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    T7 promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCellFree_G03 IL2

    Plasmid

    #67053

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL2 (IL2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTG-mTDG-K341R

    Plasmid

    #52267

    Purpose

    Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K341R, SUMO acceptor site mutation

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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