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GLN, Linear-3, PolyU(4)

Plasmid

#166985

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

Plasmid

#166995

Purpose

Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2365 - Intron GG1 - 250bp no PATC

Plasmid

#159883

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2345 - Intron GG2 - 250bp no PATC

Plasmid

#159884

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2366 - intron GG3 - 250bp no PATC

Plasmid

#159885

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp no PATC

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N0

Plasmid

#160983

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1-2A

Plasmid

#160986

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2-2A

Plasmid

#160987

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2

Plasmid

#160985

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1

Plasmid

#160984

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-JNKKTR -miRFP670-WPRE

Plasmid

#159976

Purpose

single wavelength translocating reporter of the JNK pathway

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

JNKKTR -miRFP670

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn

Plasmid

#159503

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn

Plasmid

#159493

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(7)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn

Plasmid

#159490

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(4)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn

Plasmid

#159488

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(2)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge