We narrowed to 9,384 results for: Coli

1. pQE80_T5_P9-ehaA_LacI

   Plasmid

   #186317

   Purpose

   Plasmid for inducible E coli expression of the P9 helixCAM.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Insert

   P9-ehaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE80_T5_AP10-ehaA_LacI

   Plasmid

   #186318

   Purpose

   Plasmid for inducible E coli expression of the AP10 helixCAM.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Insert

   AP10-ehaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA5136

   Plasmid

   #184853

   Purpose

   A retroviral vector encoding hygromycin resistance and PhiC31 recombinase

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Insert

   PhiC31o recombinase
   

   Use

   Retroviral; Phic31 attp-attb recombination/excisi…

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   LTR

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b_SNAP-ecDHFRmt

   Plasmid

   #187109

   Purpose

   Expression of SNAP-ecDHFRmt fusion in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   SNAP-ecDHFRmt
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   ecDHFR-R44L-H45Q

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-1b-S10

   Plasmid

   #128594

   Purpose

   Expresses tagless S10 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S10 (rpsJ E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSF-1b-S3

   Plasmid

   #128591

   Purpose

   Expresses tagless S3 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   S3 E.coli ribosomal protein (rpsC E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

   Plasmid

   #170767

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XbaI
   M.EcoRI
   M.SalI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSETA_crG18

   Plasmid

   #172930

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSG

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorG18
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET19b-HisDAD2

    Plasmid

    #165996

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQDKN0329

    Plasmid

    #162315

    Purpose

    TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-NET-TEVprotease-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CM0548

    Plasmid

    #162299

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0KN0244

    Plasmid

    #162282

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0KN0024

    Plasmid

    #162281

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPQD0CM0539

    Plasmid

    #162306

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, TEV protease (S219V), no stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S219V, codon optimized for E. coli

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0CM0547

    Plasmid

    #162298

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0CM0546

    Plasmid

    #162297

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPQD0CM0545

    Plasmid

    #162296

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQD0CM0544

    Plasmid

    #162295

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQD0CM0543

    Plasmid

    #162294

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge