164,802 results

1. TIGRE docking site targeting vector

   Plasmid

   #61580

   Purpose

   Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB512

   Plasmid

   #74854

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. iRFP-H2A

   Plasmid

   #158691

   Purpose

   This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mAzurite-C1

   Plasmid

   #54583

   Purpose

   Localization: C1 Cloning Vector, Excitation: 384, Emission: 450

   Depositor

   Patrick Daugherty, Michael Davidson

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Type

   Empty backbone

   Use

   Tags

   mAzurite

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. GFP-EEA1 wt

   Plasmid

   #42307

   Depositor

   Silvia Corvera

   Article

   Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2/BH

   Plasmid

   #26556

   Depositor

   Perry Hackett

   Insert

   pT2/BH
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS_3E5E_eGFP

   Plasmid

   #103054

   Purpose

   Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebola virus (ebolavirus Zaire, Mayinga) minigenome
   

   Use

   Tags

   3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rosa26-LSL-PE2 Targeting Vector

    Plasmid

    #196038

    Purpose

    The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    Cas9-MMLV-RT
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBE45

    Plasmid

    #174655

    Purpose

    CAPTURE method receiver plasmid for Streptomyces heterologous expression

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-GFP-Kir2.1

    Plasmid

    #107184

    Purpose

    mammalian expression of Kir2.1

    Depositor

    Bikash Pattnaik

    Insert

    KCNJ2 (KCNJ2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-Cepheid1b-ST-WPRE

    Plasmid

    #214967

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

    Depositor

    Peng Zou

    Article

    Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

    Insert

    Cepheid1b-ST
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBE44

    Plasmid

    #174654

    Purpose

    CAPTURE method 15A origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge