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Actin-BAF53(a+b)-ires-EGFP

Plasmid

#25461

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53(a+b)

Tags

EGFP

Expression

Mammalian

Mutation

3' of mBAF53a deleted and fused to 3' o…

Available Since

Sept. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Promoter

pLuxR

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRF032d

Plasmid

#156353

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKB255

Plasmid

#170038

Purpose

Encodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Melittin

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pOX + kqt2

Plasmid

#16137

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-2 Nematode)

Use

Xenopus oocyte expression

Mutation

Deletion of aa1-38 compared to GenBank NP_509392.3

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pL0_AC_p45582

Plasmid

#154036

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter 45582

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tNub

Plasmid

#154039

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Nub

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_AC_pSVP

Plasmid

#154040

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter SVP

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tPbt

Plasmid

#154043

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Pbt

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pstop

Plasmid

#109066

Purpose

inserts a polyA site to end transcription of a targetted gene

Depositor

Eric Lander

Article

Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pC220

Plasmid

#65877

Purpose

Medium P2 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Expression

Bacterial

Mutation

LAA tagged

Promoter

Prhl/lac

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJB044

Plasmid

#22617

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

Human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pCB6-ZONAB-B

Plasmid

#136642

Purpose

Expression of ZONAB isoform B in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform B (YBX3 Canine)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(0.4)-R5

Plasmid

#32578

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 0.4kb version

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pProm9_BCD1-GFP

Plasmid

#80655

Purpose

Promoter9 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJL042

Plasmid

#122865

Purpose

Binary expression vector containing 35S:MS2-KYP:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-KYP:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL032

Plasmid

#122855

Purpose

KYP SET domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SET catalytic domain of KYP H3K9 histone methyltransferase

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL035

Plasmid

#122854

Purpose

G9a SET domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

G9a SET catalytic domain, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJL023

Plasmid

#122841

Purpose

p300 core domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Core domain of p300 H3K27 histone acetyltransferase, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMH186

Plasmid

#122837

Purpose

MS2-NLS (no stop codon) with GreenGate C-D flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-NLS, codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Tags

Nuclear localisation signal

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJB036

Plasmid

#22624

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (PLP1 Human)

Use

Cat reporter plasmid

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_hM3O

Plasmid

#112638

Purpose

Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hM3O

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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RV 2-5

Plasmid

#68410

Purpose

Retroviral backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Retroviral; Gmap

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-R4r-LucmiR-EGFP-R3r

Plasmid

#32589

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Intronic Luciferase miRNA and EGFP

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pRS426-ACT_unstable

Plasmid

#127608

Purpose

high-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ACT1 intron (WT)

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_stable

Plasmid

#127606

Purpose

high-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ECM33 intron (WT)

Expression

Yeast

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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PRRX2-pBLSK

Plasmid

#21012

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

PRRX2 (Prrx2 Mouse)

Expression

Bacterial

Mutation

Missing first three amino acids at N-terminus

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pCM3.06

Plasmid

#19602

Depositor

Geraldine Seydoux

Article

Lee et al PLoS Genet. 2007 Dec 28. 3(12):e233.

Insert

fbf-1 ORF+3' in pDONR201 (fbf-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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