We narrowed to 9,184 results for: Pol;

1. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131462

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A K27M, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131461

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131463

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A G34R, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-TetOminiCMV-OSKM

   Plasmid

   #136541

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-OKMS

   Plasmid

   #80480

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-OK+9MS

   Plasmid

   #80482

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

   Plasmid

   #129420

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   TagBFP2-V5 tag

   Mutation

   G12V

   Promoter

   none (3x polyA to mitigate episomal expression)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1280

   Plasmid

   #29142

   Purpose

   Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple249 (VIP Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1281

    Plasmid

    #29143

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple250 (VIP Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEMS1279

    Plasmid

    #29141

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple248 (VIP Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEMS1722

    Plasmid

    #29298

    Purpose

    Expression of the reporter gene was not detected in the brain and eye using LacZ.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple250 (VIP Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    June 10, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. FUW-tetO-hOKMS

    Plasmid

    #51543

    Purpose

    Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

    Depositor

    Tarjei Mikkelsen

    Article

    Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

    Insert

    hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TAC-EB-C5

    Plasmid

    #80485

    Purpose

    piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS1382

    Plasmid

    #29182

    Purpose

    Expression of the eGFP reporter gene was detected (positive) in the brain, but not detected (negative) in the eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple183 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEMS1385

    Plasmid

    #29184

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple186 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS1383

    Plasmid

    #29183

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple184 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1102

    Plasmid

    #29033

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple183 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEMS1103

    Plasmid

    #29034

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple184 (S100B Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Expression

    Mammalian

    Available Since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS-GBX2-CDX124-Ef1a-mCherry-Neo

    Plasmid

    #192823

    Purpose

    Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFBOH-LIC_DDA1:1-102

    Plasmid

    #210902

    Purpose

    Insect expression of full-length human DDA1. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DDA1:M1-T102 (DDA1 Human)
    

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pFBD-BirA_RACK1:1-317

    Plasmid

    #210889

    Purpose

    Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RACK1:M1-R317 (RACK1 Human)
    

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pFBOH-LIC_SMU1:1-513

    Plasmid

    #210877

    Purpose

    Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SMU1:M1-P513 (SMU1 Human)
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pBiT3.1-N_DCAF8L2:1-631

    Plasmid

    #211239

    Purpose

    Mammalian expression of full-length human DCAF8L2. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF8L2:M1-S631 (DCAF8L2 Human)
    

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    145-147: missing in Poly E region

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pFBOH-LIC_MAGEA3:104-314

    Plasmid

    #210941

    Purpose

    Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    MAGEA3:E104-E314 (MAGEA3 Human)
    

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pFBOH-MHL_UTP15:1-370

    Plasmid

    #210937

    Purpose

    Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    UTP15:M1-H370 (UTP15 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pFBOH-MHL_TBL2:70-405

    Plasmid

    #210936

    Purpose

    Insect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    TBL2:E70-H405 (TBL2 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pFBOH-MHL_SHKBP1:182-585

    Plasmid

    #210935

    Purpose

    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SHKBP1:T182-L585 (SHKBP1 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    Q507L natural variant (VAR_036714)

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge