We narrowed to 9,384 results for: Coli

1. pTH1-SaSrtA-A

   Plasmid

   #64979

   Purpose

   Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108A

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH1-SaSrtA-K

   Plasmid

   #64978

   Purpose

   Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-p53 (Cow 1-82)

   Plasmid

   #62062

   Purpose

   Expression of Cow p53 (residues 1-82) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-82

   Promoter

   T7

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET28-p53 (Rabbit 1-87)

   Plasmid

   #62058

   Purpose

   Expression of Rabbit p53 (residues 1-87) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-87

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pHL1506

   Plasmid

   #53026

   Purpose

   PLlacO-1:RBS(st7):csrA

   Depositor

   Han Lim

   Article

   Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

   Insert

   csrA
   

   Use

   Synthetic Biology

   Tags

   RBS(st7)

   Expression

   Bacterial

   Mutation

   Promoter

   pLlac0-1

   Available since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET-28a-CylI

   Plasmid

   #48200

   Purpose

   Expresses CylI (type III PKS) in E. coli

   Depositor

   Emily Balskus

   Article

   Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

   Insert

   CylI
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-29b-CylB

   Plasmid

   #48199

   Purpose

   Expresses CylB (acyl carrier protein) in E. coli

   Depositor

   Emily Balskus

   Article

   Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

   Insert

   CylB
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p70rg 11

   Plasmid

   #17830

   Depositor

   Jay Keasling

   Article

   Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

   Insert

   E. coli optimized red flourescent protein (RFPec), GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pAKW.114A-PKA

   Plasmid

   #154051

   Purpose

   Expresses PKA in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pCin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His10-Draper delta ITAM

    Plasmid

    #126671

    Purpose

    Expression of His10-Draper delta ITAM mutant for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic ITAM residues mutated to Phe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic ITAM residues mutated to Phe

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid

    #209086

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid

    #209085

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEvolvR-enCas9-PolI3M-TBD

    Plasmid

    #113077

    Purpose

    Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI3M-TBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCT5-bac1.8

    Plasmid

    #119871

    Purpose

    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR'
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    SNP in 592 bp

    Promoter

    PserA promoter and Pveg promoter with CuO sequence

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCT5-bac2.0

    Plasmid

    #119872

    Purpose

    Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

    Depositor

    Claudia Schmidt-dannert

    Article

    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

    Inserts

    CymR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg promoter with CuO sequence and PxylR promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Use

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHB-4

    Plasmid

    #140957

    Purpose

    For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pet-MBP

    Plasmid

    #209184

    Purpose

    Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Type

    Empty backbone

    Use

    Tags

    HisX6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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