We narrowed to 14,023 results for: Egf

1. pEGFP Kind2 S159/181/666E

   Plasmid

   #105314

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666E

   Promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Akt2(K14A/R25E)-Halo-PS

   Plasmid

   #86632

   Purpose

   Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A/R25E)-Halo-PS (AKT2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

   Plasmid

   #84253

   Purpose

   Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

   Plasmid

   #84252

   Purpose

   Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pES006 (pTet-qacR 2/pQacA-deGFP)

   Plasmid

   #69035

   Purpose

   reporter with qacR 2 on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

   Promoter

   pQacA and pTet

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pES007 (pTet-qacR 5/pQacA-deGFP)

   Plasmid

   #69036

   Purpose

   reporter with qacR 5 on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   W61Y, E90Q, F102S, M116Q, Y119L, E120Q

   Promoter

   pQacA and pTet

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Enh eGFP Reporter-muKC2-X7

   Plasmid

   #59231

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt5-ex3-5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Dec. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLV-Enh eGFP Reporter-muKC2-X8

   Plasmid

   #59232

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt5-ex7
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLV-Enh eGFP Reporter-muKC2-X5

   Plasmid

   #59229

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt5 ex1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLV-Enh eGFP Reporter-muKC2-X4

    Plasmid

    #59228

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt4-ex7-9 and 3' UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLV-Enh eGFP Reporter-muKC2-X6

    Plasmid

    #59230

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt5 ex2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLV-Enh eGFP Reporter-muKC2-X3

    Plasmid

    #59227

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt4-ex7-9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #125691

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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