We narrowed to 10,402 results for: plasmids 101

1. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sox2-t2A-GFP

   Plasmid

   #127537

   Purpose

   Expresses human SOX2 and eGFP via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   SOX2 (SOX2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-memRFP-3xPax7gRNA

   Plasmid

   #224569

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sox2-t2A-mCherry

   Plasmid

   #127538

   Purpose

   Expresses human SOX2 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   SOX2 (SOX2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

   Plasmid

   #159894

   Purpose

   AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

   Depositor

   Tatsushi Onaka

   Article

   Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

   Insert

   NES-tdTomato-LINuS
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1α promoter

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM90

   Plasmid

   #226682

   Purpose

   Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

   Depositor

   Andreas Martin

   Article

   Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

   Inserts

   Rpn5 (RPN5 Budding Yeast)
   Rpn6 (RPN6 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   Rpn9 (RPN9 Budding Yeast)
   Rpn11 (RPN11 Budding Yeast)
   

   Tags

   MBP-HRV

   Expression

   Bacterial

   Mutation

   S111TAG

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pArk313

   Plasmid

   #200257

   Purpose

   Ark313 rep/cap

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   Ark313 Rep/Cap
   

   Use

   AAV

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-PGK-ABI-dCas9-P2A-mcherry

   Plasmid

   #121513

   Purpose

   expression of AB1-fused-dcas9 plasmids

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   AB1 and P2A-mcherry

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. plRL117

   Plasmid

   #163635

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HDM-Hgpm2

    Plasmid

    #204152

    Purpose

    Lentivirus helper plasmid coding for Gag/Pol

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    HIV-1 gag/pol
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HDM-tat1b

    Plasmid

    #204154

    Purpose

    Lentivirus helper plasmid coding for Tat

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    tat
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Tags

    mNeon

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

    Plasmid

    #230988

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1 gfaABC1D-cyto-GCaMP6f

    Plasmid

    #52925

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Article

    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

    Has Service

    AAV5

    Insert

    GCaMP6f
    

    Use

    AAV

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Expression

    Mammalian

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-EGFP-WPRE

    Plasmid

    #187103

    Purpose

    Double floxed EGFP under the control of EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hsyn-GRAB_HA1m

    Plasmid

    #190474

    Purpose

    Express the medium-affinity histamine sensor GRAB_HA1m in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1m (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pP2A_NLS_sfGFP

    Plasmid

    #63709

    Purpose

    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    

    Use

    Just a donor dna with nanog homologous arm for kn…

    Tags

    sfGFP

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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