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ATF4 14: 5’ATF4.uORF1&2AUA.GFP

Plasmid

#21861

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codons of uORFs1&2 were converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pRDAX-395 HA miRFP670[1] IRES miRFP670[2]

Plasmid

#130143

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

miRFP670

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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BKbeta2 K+ channel scFv [N53/32]

Plasmid

#190561

Purpose

Mammalian Expression of BKbeta2 K+ channel scFV. Derived from hybridoma N53/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

BKbeta2 K+ channel (Mus musculus) recombinant scFV (Kcnmb2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-B9D1-N-18

Plasmid

#54011

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

B9D1 (B9d1 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Nanog-pA-pgk-hph

Plasmid

#74915

Purpose

Mammalian expression of mNanog

Depositor

Jose Silva

Article

Theunissen et al Curr Biol. 2011 Jan 11;21(1):65-71. doi:

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-p85beta

Plasmid

#1406

Depositor

Lewis Cantley

Insert

PI3K regulatory subunit p85beta (Pik3r2 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
Smad3-shRNA

Plasmid

#32806

Depositor

Vicki Boussiotis

Insert

Smad3 (Smad3 Mouse)

Use

RNAi

Tags

DsRed

Expression

Mammalian

Promoter

hU6

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCR4-MBII52-probe

Plasmid

#67647

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII52 snoRNA (Snord115 Mouse)

Use

For in vitro transcription

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pAPM sh2 DNMT1

Plasmid

#88885

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.2 DNMT1

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV (spleen focus forming virus)

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y576/577F

Plasmid

#50505

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y576/577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

Plasmid

#196896

Purpose

Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

N-Cadherin-2xHA (Cdh1 Mouse)

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean-H3.3-N-14

Plasmid

#55377

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Insert

H3.3 (H3f3b Mouse)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10]

Plasmid

#190311

Purpose

Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

Plasmid

#174134

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Cav1 in situ probe

Plasmid

#45624

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Cav1 in situ probe (Cav1 Mouse)

Use

In situ

Mutation

fragment contains bp# 1472-2124 of AB029929.1

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
Anti-WRN [N428/12R]

Plasmid

#206575

Purpose

Mammalian Expression Plasmid of anti-WRN (Human). Derived from hybridoma N428/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-WRN (Homo sapiens) recombinant Mouse monoclonal antibody (WRN Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Neuregulin-CRD (Cysteine-rich domain, Type III) scFv [N126B/31]

Plasmid

#190528

Purpose

Mammalian Expression of Neuregulin-CRD (Cysteine-rich domain, Type III) scFV. Derived from hybridoma N126B/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant scFV (NRG1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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