We narrowed to 15,723 results for: LEA

1. pICSL86977OD:PITG_04085

   Plasmid

   #173509

   Purpose

   This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

   Depositor

   Sophien Kamoun

   Article

   Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

   Insert

   PITG_04085_T30-4
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL86977OD:PITG_02860

   Plasmid

   #173508

   Purpose

   This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

   Depositor

   Sophien Kamoun

   Article

   Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

   Insert

   PITG_02860_T30-4
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICSL86977OD:PITG_02860a

   Plasmid

   #173507

   Purpose

   This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

   Depositor

   Sophien Kamoun

   Article

   Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

   Insert

   PITG_02860_P17777
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL86977OD:PITG_00582

   Plasmid

   #173506

   Purpose

   This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

   Depositor

   Sophien Kamoun

   Article

   Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

   Insert

   PITG_00582_T30-4
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL N-Myc-hPOT1_K90E-IRES_hygro

   Plasmid

   #166407

   Purpose

   Express hPOT1 K90E in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K90E

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc227

   Plasmid

   #161948

   Purpose

   Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. IBMc367

   Plasmid

   #161947

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. IBMc228

   Plasmid

   #161946

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-GFP-EBA175-Flag(P1424G)

    Plasmid

    #155128

    Purpose

    Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424G; insert codes for EBA175 residues 1284 to …

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-HA-PfROM4 (H578A)

    Plasmid

    #154858

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Histidine 578 changed to Alanine

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid

    #154859

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Glycine 470 changed to Alanine and Glycine 471 ch…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15-His-∆NGlpG(C104A/M247C)

    Plasmid

    #155133

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/M247C, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM5

    Plasmid

    #154166

    Purpose

    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM28

    Plasmid

    #154165

    Purpose

    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel1A
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMtb MCM

    Plasmid

    #138296

    Purpose

    Expression of Mtb MCM small and large subunit

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Inserts

    mutA
    mutB
    

    Tags

    His tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    inserted TEV cleavage site between His-tag and m…

    Promoter

    T7

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK353

    Plasmid

    #129697

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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