We narrowed to 15,601 results for: LEA

1. pDEST-DHFR F[3]-C (LEU2)

   Plasmid

   #177796

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[3]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5-Flag-XBP1s

   Plasmid

   #63680

   Purpose

   Mammalian expression of Flag tagged XBP1s

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLmMBP-10

   Plasmid

   #72348

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_iPE-N

   Plasmid

   #214734

   Purpose

   encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-N_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG_iPE-C

   Plasmid

   #214735

   Purpose

   encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-C_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZT-C13-R1

   Plasmid

   #62197

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Right CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIP-SFFV-tagBFP-2A-ICP0-FxE

   Plasmid

   #235539

   Purpose

   To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

   Insert

   ICP0-FxE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of the RING domain, ∆106-149.

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZT-C13-L1

   Plasmid

   #62196

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Left CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

   Plasmid

   #231986

   Purpose

   Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   OVA-2A-ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-SFB-DYRK1A

    Plasmid

    #101770

    Purpose

    Mammalian expression of DYRK1A fusion protein

    Depositor

    Michael Huen

    Article

    Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLIB-WDR61

    Plasmid

    #231729

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    WDR61 (SKIC8 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG1-PHP.eB

    Plasmid

    #220799

    Purpose

    AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    E4, E2A, VA, AAV2 rep and PHP.eB cap genes
    

    Use

    AAV; Aav helper vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-SUMO-Rad23A_pET11a

    Plasmid

    #169124

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hRad23A (RAD23A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-Cre

    Plasmid

    #55636

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV11

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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