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pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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mChT pcDNA3

Plasmid

#15765

Depositor

Randy Blakely

Article

Apparsundaram et al Biochem Soc Trans. 2001 Nov . 29(Pt 6):7

Insert

hemicholinium-3-sensitive choline transporter (Slc5a7 Mouse)

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pDEST27-Vinculin

Plasmid

#20144

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pDEST27-Vinculin (Vcl Mouse)

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pCRISPaint-HA

Plasmid

#67171

Purpose

CRISPaint universal donor for HA fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HA

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD3-mCardinal-nat

Plasmid

#120333

Purpose

Bacterial mCardinal promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mCardinal

Expression

Bacterial

Promoter

(None)

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Expression

Bacterial

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNHP

Plasmid

#22500

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

gag, pol, tat, rev

Expression

Mammalian

Available Since

March 12, 2010

Availability

Academic Institutions and Nonprofits only
pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMAP L2B

Plasmid

#153357

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pYH80

Plasmid

#217387

Purpose

Ptrc99A-mCherry-L78-PopTag for bacterial expression

Depositor

Anthony Vecchiarelli

Article

Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

Insert

PopTag

Tags

mCherry-Linker78

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFH22

Plasmid

#125808

Purpose

Level0 xCas9 3.7

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

xCas9 3.7

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-TRP1

Plasmid

#26877

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-TRP1

Plasmid

#26874

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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GNb-SNAPf

Plasmid

#128770

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-SNAPf

Expression

Mammalian

Available Since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-B0034m-E0040m-B0015)

Plasmid

#197659

Purpose

Expresses GFP under the control of J23100 and B0034m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB-TB

Plasmid

#36099

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pClodANL-Cherry

Plasmid

#107870

Purpose

Bacterial expression of cherry-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

Cherry (red fluorescent protein)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Expression

Mammalian

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b-NpR5600

Plasmid

#45741

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

NpR5600 (Npun_R5600 Nostoc punctiforme)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only