We narrowed to 10,402 results for: plasmids 101

1. pJZC588

   Plasmid

   #62315

   Purpose

   sgRNA with 2x MS2 (wt+f6) for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x MS2 (wt+f6) binding module
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJZC518

   Plasmid

   #62277

   Purpose

   Yeast dCas9 expression plasmid

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   dCas9
   

   Expression

   Yeast

   Mutation

   Has mutations in the RuvC1 and HNH domains to ren…

   Promoter

   pTdh3

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPR_DMS3g24

   Plasmid

   #89244

   Purpose

   Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   CRISPR
   

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGTPA-lb

   Plasmid

   #171528

   Purpose

   Plasmid contains the Cas12k gRNA for mutant library construction

   Depositor

   Quanjiang Ji

   Article

   Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

   Insert

   Cas12k gRNA
   

   Use

   CRISPR

   Promoter

   Constitutive promoter J23119

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGTPA

   Plasmid

   #171525

   Purpose

   Plasmid contains the Cas12k gRNA and transposon donor cassette

   Depositor

   Quanjiang Ji

   Article

   Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

   Insert

   Cas12k gRNA, transposon donor cassette
   

   Use

   CRISPR

   Promoter

   Constitutive promoter J23119

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-L7-6-DD-T6B-WT-YFP

   Plasmid

   #235145

   Purpose

   Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   DHFR-fused T6B peptide
   

   Use

   AAV

   Promoter

   L7-6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-L7-6-FLAG-HA-T6B-WT-YFP

   Plasmid

   #235141

   Purpose

   Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   T6B peptide
   

   Use

   AAV

   Tags

   FLAG/HA

   Promoter

   L7-6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSNRW-Z3-Nluc

   Plasmid

   #174486

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z3-Nluc
   

   Tags

   6x Histidine tag

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EED-KO-gRNA-2

   Plasmid

   #131322

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

10. KG#493

    Plasmid

    #110932

    Purpose

    Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

    Depositor

    Kenneth Miller

    Article

    Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

    Inserts

    unc-17 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KG#94

    Plasmid

    #110931

    Purpose

    Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

    Depositor

    Kenneth Miller

    Article

    Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

    Inserts

    unc-17 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230989

    Purpose

    Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHiUGE smFP-V5 donor ORF0

    Plasmid

    #200380

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHiUGE smFP-V5 donor ORF1

    Plasmid

    #200381

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge