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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human alpha5 ecto

Plasmid

#221394

Purpose

Mammalian expression of human integrin alpha5 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 ectodomain (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HR…

Expression

Mammalian

Mutation

codon optimized for human mature residues _5 F1 t…

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_832

Plasmid

#216085

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-TTI1 zeo

Plasmid

#192328

Purpose

Lentiviral expression vector for an inducible 3xFLAG-TTI1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

TTI1 (TTI1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_2

Plasmid

#135760

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_1

Plasmid

#135759

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-637.sensor.PGK.dNGFR.WPRE

Plasmid

#85873

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-637 Sensor (MIR637 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-572.sensor.PGK.dNGFR.WPRE

Plasmid

#85914

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-572 Sensor (MIR572 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-942-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85888

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-942-3p Sensor (MIR942 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-920.sensor.PGK.dNGFR.WPRE

Plasmid

#85924

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-920 Sensor (MIR920 Human)

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-224-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85899

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-224-3p Sensor (MIR224 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-181c-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85891

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-181c-5p Sensor (MIR181C Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGluSnFR4s-NGR-WPRE

Plasmid

#234451

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4s-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6085x

Plasmid

#108653

Purpose

A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

PicchuEV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pp-CBBC-Fluc

Plasmid

#232920

Purpose

Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.

Depositor

Philippe Mangeot

Article

Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_NTRK3

Plasmid

#205817

Purpose

Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_NTRK3 (ETV6, NTRK3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-natMX6 (pFB1434)

Plasmid

#126050

Purpose

For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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