We narrowed to 1,590 results for: PTS

1. pJET-67-DExCon-mNeonGreen-R11b

   Plasmid

   #179899

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJET-59-DExCon-mTagBFP2-R25

   Plasmid

   #179900

   Purpose

   Donor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB25, member RAS oncogene family (RAB25 Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mTagBFP2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETC PITPbeta N89F

   Plasmid

   #58279

   Purpose

   bacterial expression of PITP beta N89F mutant

   Depositor

   Shamshad Cockcroft

   Article

   Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

   Insert

   PITP beta (PITPNB Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   N89F (abolishes PI binding)

   Promoter

   T7

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. GFP-MAP1B

   Plasmid

   #44396

   Depositor

   Phillip Gordon-Weeks

   Article

   Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

   Insert

   MAP1B (Map1b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP-Kif5c

   Plasmid

   #71853

   Purpose

   mammalian expression of GFP-Kif5c

   Depositor

   Michelle Peckham

   Article

   Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

   Insert

   Kif5c (Kif5c Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Kif5c starts at AA3

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. VP16-ER alpha

   Plasmid

   #11351

   Depositor

   Donald McDonnell

   Article

   Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

   Insert

   ER alpha (ESR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-FKBP-LCa

   Plasmid

   #59353

   Purpose

   Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Insert

   Clathrin Light Chain A (CLTA Human)
   

   Use

   Tags

   EGFP and FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBrain-GFP-FKBP-TACC3-shTACC3

   Plasmid

   #59354

   Purpose

   Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   EGFP and FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP-S100A10

    Plasmid

    #107200

    Purpose

    Expresses YFP-human S100A10 fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

    Insert

    S100A10 (S100A10 Human)
    

    Use

    Tags

    Yellow Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP hsp70 K71E

    Plasmid

    #15216

    Depositor

    Lois Greene

    Article

    Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

    Insert

    hsp70 K71E (HSPA1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K71E, ATPase-deficient mutant.

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSF107mT-GATEWAY-3'-FLAG

    Plasmid

    #67619

    Purpose

    A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGfa2-nLac

    Plasmid

    #53126

    Purpose

    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

    Depositor

    Michael Brenner

    Article

    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains -2163 to +47 (the human gfa2 segment), w…

    Promoter

    human GFAP

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Use

    Tags

    Halo7 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSF107mT-GATEWAY-3'-Myc tag

    Plasmid

    #67617

    Purpose

    A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N3-hARL1(WT)

    Plasmid

    #206406

    Purpose

    Mammalian expression of hARL1(WT)

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    ARL1 (ARL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSF107mT-GATEWAY-3'-3HA

    Plasmid

    #67616

    Purpose

    A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    three inframe 3' HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pOTTC414 - pAAV EF1a hPREP

    Plasmid

    #59967

    Purpose

    An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNATM3.1/V5-His TOPO-PKCi-K274W

    Plasmid

    #55690

    Purpose

    expresses dominant negative human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E and K274W

    Promoter

    CMV

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcdna DSG-2 Tailless

    Plasmid

    #89466

    Purpose

    DSG-2 tailless control

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge