Addgene

We narrowed to 5,864 results for: pcDNA 3.1

Showing: 901 - 930 of 5864 results

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pcDNA3.1his p66shc S36A

Plasmid

#10973

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

S36A

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.7 (pcDNA3.1+)

Plasmid

#75192

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Use

Tags

6xHis and mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 MYC dLZ

Plasmid

#176048

Purpose

Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Use

Tags

Expression

Mammalian

Mutation

deleted amino acids 414-433

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pY012 (pcDNA3.1-hCMtCpf1)

Plasmid

#69984

Purpose

Expresses humanized CMtCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hCMtCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-PITPalpha

Plasmid

#58277

Purpose

mammalian expression of human PITP alpha

Depositor

Shamshad Cockcroft

Insert

PITP alpha (PITPNA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Use

Tags

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Renilla-ccdB

Plasmid

#87068

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

T7 promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pF153 pcDNA3.1(+)SOD1G37R

Plasmid

#26399

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

G37R

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pY017 (pcDNA3.1-hPcCpf1)

Plasmid

#69989

Purpose

Expresses humanized PcCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPcCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-aDTX-lumitoxin

Plasmid

#51686

Purpose

Expression vector for aDTX-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

aDTX-lumitoxin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Homeodomain deleted

Promoter

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pY005 (pcDNA3.1-hLb3Cpf1)

Plasmid

#69977

Purpose

Expresses humanized Lb3Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb3Cpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNeuroguidin_AA

Plasmid

#148237

Purpose

Mammalian Expression of HsNeuroguidin

Depositor

Elisa Izaurralde

Insert

HsNeuroguidin (NGDN Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-BFP

Plasmid

#214363

Purpose

Expression of the Blue Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pY117 (pcDNA3.1-huMb3Cpf1)

Plasmid

#92293

Purpose

Expression of humanized Mb3Cpf1

Depositor

Feng Zhang

Insert

huMb3Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30A

Plasmid

#87081

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30A (Trim30a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge