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We narrowed to 171,322 results for: addgene

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Showing: 1201 - 1240 of 171322 results

  3. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  6. pTFE3.1.0-gDNA

    Plasmid
    #112473
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3
    Depositor
    Kevin White
    Insert
    TFE3 (TFE3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112473-plasmid-map-sequence-id-230504

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  12. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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  15. KEAP1 (KEAP1A-c029)

    Plasmid
    #110247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KEAP1 (KEAP1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110247-plasmid-map-sequence-id-212283

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  16. 6C1A (MBD2)

    Plasmid
    #119958
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD2 (MBD2 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119958-plasmid-map-sequence-id-232631

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  18. LEGO-AP1x6-GM55

    Plasmid
    #217415
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217415-plasmid-map-sequence-id-441062

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  23. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  24. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104404-plasmid-map-sequence-id-204454

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  25. 5WCI (MYST1)

    Plasmid
    #101269
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST1 (KAT8 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101269-plasmid-map-sequence-id-193502

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  28. pESR1.1.0-gDNA

    Plasmid
    #112433
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1
    Depositor
    Kevin White
    Insert
    ESR1 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112433-plasmid-map-sequence-id-230374

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  29. LEGO-AP1x3-GM55

    Plasmid
    #217414
    Purpose
    Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217414-plasmid-map-sequence-id-434967

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  30. ANO10 (TMEM16KA-c001)

    Plasmid
    #110267
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TMEM16K (ANO10 Human)
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    110267-plasmid-map-sequence-id-358733

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  35. CMV-Rps7-meGFP

    Plasmid
    #241318
    Purpose
    Mouse Rps7 fused to meGFP for expression in mammalian cells
    Depositor
    Stavros Lomvardas
    Insert
    Rps7 (Rps7 Mouse)
    Tags
    meGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    241318-plasmid-map-sequence-id-480861

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  36. MAPK8

    Plasmid
    #73236
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK8 (MAPK8 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 26-321
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73236-plasmid-map-sequence-id-137188

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  37. 4QQG

    Plasmid
    #61948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP:JMC001-B06:C41570 (KAT5 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pACλCI

    Plasmid
    #53730
    Purpose
    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    bacteriophage lambda CI protein
    Expression
    Bacterial
    Promoter
    lac UV5
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53730-plasmid-map-sequence-id-83908

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  40. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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Showing: 1201 - 1240 of 171322 results