1,859 results
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Plasmid#159829PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159833PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertpEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP
Plasmid#159836PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertarray co-injection fragment mosTI Pmlc-2_GFP
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA841 - hrp-1p::hrp-1HsLCWT
Plasmid#139198PurposeExpresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTRDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationlast exon replaced with correspoding human sequen…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA842 - hrp-1p::hrp-1HsLCD290V
Plasmid#139199PurposeExpresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTRDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationD290V, last exon replaced with correspoding human…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA847 - mec-4p::hrp-1mScarlet
Plasmid#139201PurposeExpresses mScarlet tagged hrp-1 in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA848 - mec-4p::hrp-1HsLCWTmScarlet
Plasmid#139202PurposeExpresses mScarlet tagged hrp-1HsLCWT in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA849 - mec-4p::hrp-1HsLCD290VmScarlet
Plasmid#139203PurposeExpresses mScarlet tagged hrp-1HsLCD290V in mec-4 neuronsDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA844 - hrp-1p::hrp-1mScarlet
Plasmid#139204PurposeExpresses mScarlet tagged hrp-1DepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA845 - hrp-1p::hrp-1HsLCWTmScarlet
Plasmid#139205PurposeExpresses mScarlet tagged hrp-1HsLCWTDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, last exon rep…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet
Plasmid#139206PurposeExpresses mScarlet tagged hrp-1HsLCD290VDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LC, D290V, last e…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJ4M_HRP-1_FL
Plasmid#139117Purposeexpress HRP-1 with a C-terminal maltose binding protein tagDepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHJ4
Plasmid#158785PurposePan-neuronal nuclear-localized GCaMP expression in C. elegansDepositorInsertPrab-3::nls::GCaMP6m
UseTagsNLSExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1884
Plasmid#154332PurposesgRNA Target Vector for CRISPRDepositorInsertcxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1827
Plasmid#154323PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1841
Plasmid#154338PurposePromoter reporterDepositorInsertSV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
UseTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1948
Plasmid#154340PurposeEnhancer reporterDepositorInsertpes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
UseTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD289
Plasmid#137018PurposeGFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized GFPExpressionWormMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDD376
Plasmid#137022PurposeSapTrap mKate2-GLO donorDepositorInsertC. elegans GLO-optimized mKate2
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized mKate2ExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF90
Plasmid#139044PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag::HDEL (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY921
Plasmid#135092PurposeTranscriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
UseTagsGFP and Synthetic IntronExpressionWormMutationPromoterPacr-2Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2750
Plasmid#135094PurposeSite specific CRISPR/Cas9 editing of C. elegans Chr IVDepositorInsertsgRNA for cxTi10082 (actgttggatgcctgtgtag)
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorInsertMyo 9 (hum-7 Nematode)
UseTagsAvi and FlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Unc104_K560GFP
Plasmid#129773PurposeExpresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertUnc104 (unc-104 Nematode)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 361, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJPL3[flp-6p::cmk-1::GFP]
Plasmid#124613PurposeUsed to confirm the expression of the trans-spliced GFP reporter in the expected ASE neuronsDepositorInsert[flp-6p::cmk-1::GFP]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC684
Plasmid#107022PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifsDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC682
Plasmid#107020PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motifDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS2-his-24
Plasmid#124318PurposeVector Backbone for his-24 fusion and let-858 3'UTRDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24
Plasmid#124317PurposeVector Backbone for his-24 fusion and unc-54 3'UTRDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::mOrange
Plasmid#124346PurposeNuclear Localized Pan-Neuronal mOrangeDepositorInsertrab-3, mOrange (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::GCaMP6s
Plasmid#124333PurposeExpressing Nuclear Localized GCaMP6sDepositorInsertGCaMP6s (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::mNeptunr
Plasmid#124331PurposeExpressing Nuclear Localized mNeptuneDepositorInsertmNeptune (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS2-his-24::mCherry
Plasmid#124328PurposeExpressing Nuclear Localized mCherryDepositorInsertmCherry (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::mCherry
Plasmid#124327PurposeExpressing Nuclear Localized mCherryDepositorInsertmCherry (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only