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pHAGE-eGFP-TAX1BP1 CC1D

Plasmid

#175774

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC1D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4 rat NZAP

Plasmid

#45908

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

Zc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only
Hoxd10 probe

Plasmid

#34712

Depositor

Ellen Carpenter

Article

Choe et al Gene Expr Patterns. 2006 Oct;6(7):730-7.

Insert

Hoxd10 (Hoxd10 Mouse)

Use

In situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218637

Purpose

Proximity biotinylation control of OSBP(PH domain)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

OSBP (OSBP Human)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

PH domain only

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g2 LentiCRISPRv2-mCherry

Plasmid

#218663

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218634

Purpose

Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

delPYD (I125M-END)

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g1 LentiCRISPRv2-mCherry

Plasmid

#218662

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-FAM134C-GFP

Plasmid

#201932

Purpose

PiggyBac Vector expressing FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-TEX264ΔLIR-GFP

Plasmid

#201930

Purpose

PiggyBac Vector expressing TEX264ΔLIR-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

F273A

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 V192S

Plasmid

#175769

Purpose

Lentiviral vector expressing N-terminally tagged GFP-TAX1BP1 V192S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

V192S

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 Y635A

Plasmid

#175772

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y635A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 N637A

Plasmid

#175773

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

N637A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD

Plasmid

#235577

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

Plasmid

#235586

Purpose

Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Tags

Expression

Mutation

G282A

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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