We narrowed to 31,835 results for: Eng;

1. pDSM-de

   Plasmid

   #127720

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSM-ef

   Plasmid

   #127721

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSM-f3

   Plasmid

   #127722

   Purpose

   Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Availability

   Academic Institutions and Nonprofits only

   

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4. negActinin-sstFRET-GR

   Plasmid

   #83417

   Purpose

   FRET-based actinin tension sensor (negative control)

   Depositor

   Tetsuya Kitaguchi

   Article

   Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

   Insert

   negActinin-sstFRET
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

   Plasmid

   #169524

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT1

   Plasmid

   #98299

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
   

   Expression

   Yeast

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL-c5x_UnaG

   Plasmid

   #163125

   Purpose

   A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   UnaG
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScPCK1

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ScADH2

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2374-Tier1-PhCMV-Gal4_VP16

    Plasmid

    #169582

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    GAL4-transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPC019.AAV

    Plasmid

    #171147

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPMVAd3

    Plasmid

    #98298

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

    Plasmid

    #169558

    Purpose

    Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PSV40-driven citrine-P2A-SEAP fusion construct
    

    Expression

    Mammalian

    Promoter

    PSV40

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Expression

    Yeast

    Available Since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. NFkBr-IL1Ra

    Plasmid

    #183065

    Purpose

    Expresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathway

    Depositor

    Farshid Guilak

    Article

    Pferdehirt et al Tissue Eng Part A. 2019 May;25(9-10):809

    Insert

    interleukin-1 receptor antagonist (Il1rn Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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