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pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45 (delta 154–164)
Plasmid#126879PurposeExpresses crystallisation construct of human Cdc45 in bacteriaDepositorInsertHuman Cdc45 (CDC45 Human)
UseTagsNo tagExpressionBacterialMutationdeletion amino acid 154-164PromoterT7 promoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLA CMV N-Flag RALB V23 R47
Plasmid#50983PurposeExpresses RALB-V23/R47 in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsFlagExpressionMammalianMutationG23V, K47RPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLA CMV N-Flag RALB V23 R160
Plasmid#50982PurposeExpresses RALB-V23/R160 in mammalian cellsDepositorInsertRALB (RALB Human)
UseLentiviralTagsFlagExpressionMammalianMutationG23V K160RPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1241
Plasmid#29123PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle12
UseTagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1932
Plasmid#79781PurposeHigh copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insertDepositorInsertPle259
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterPle259 (PAX6 MiniPromoter)Available sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1937
Plasmid#79782PurposeHigh copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insertDepositorInsertPle260
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterPle260 (PAX6 MiniPromoter)Available sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCK036 desmaMCS:minCerulean,cryaa:Venus
Plasmid#195981PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
Plasmid#159817PurposePositive control for germline GFP expression from simple array in C. elegansDepositorInsertpEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
TurboID-CRaf
Plasmid#226168PurposeLentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.DepositorInsertMyc-TurboID-CRaf (RAF1 Human)
UseLentiviralTagsEGFP-IRES-BlastR and Myc-TurboIDExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA1-28-pA
Plasmid#55200PurposePlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARdeltaNH2-6
Plasmid#89104Purposemammalian expression of human androgen receptor amino acids 507-919DepositorInserthAR DNA binding domain+ligand binding domain (AR Human)
UseTagsExpressionMammalianMutationNH2 terminal deletion, contains amino acids 507-…PromoterCMVAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-TVA-P2A-N2cG
Plasmid#172363PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinaseDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK033 p5E desma MCS
Plasmid#195948PurposeGateway p5E vectorDepositorInsertdesmin a regulatory elements (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP (empty vector)
Plasmid#172604PurposeEmpty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cellsDepositorTypeEmpty backboneUseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T373
Plasmid#58912Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T373 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK069 desmaMCS:minprom_mApple,exorh:EGFP
Plasmid#195982PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only
