We narrowed to 14,207 results for: RING;

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pCI108-SiT-Cas12a-[Cond]

Plasmid

#128407

Purpose

Conditionally express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2B

Plasmid

#23998

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2B (Grin2b Rat)

Tags

NR2B signal sequence (aa1-31) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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His-CL7-2NLS-SpyCas9(dS)-A22p3star

Plasmid

#222970

Purpose

Bacterial expression of A22p-fused SpyCas9 construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His-CL7-2NLS-SpyCas9(dS)-A22p3

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pARF6-CFP

Plasmid

#11382

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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PAC_E83_LOV_eGFP

Plasmid

#231574

Purpose

This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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mEGFP-Cdc42-C1

Plasmid

#29673

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

Cdc42 (CDC42 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209724

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-Control

Plasmid

#122277

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Bacterial

Promoter

Thymidine kinase

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Tags

SEP

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCggRO-reporter

Plasmid

#124582

Purpose

Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

CggR promoter element

Expression

Yeast

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
pCDB179-DepoPETase

Plasmid

#218702

Purpose

Encodes codon optimized DepoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

DepoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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