We narrowed to 9,085 results for: mel

1. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX307-SARS-CoV-3CL

   Plasmid

   #160280

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MED4

   Plasmid

   #15425

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED4 (MED4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMOS009: pLX304-QuasAr2

   Plasmid

   #62985

   Purpose

   Lentivirus production of QuasAr2 fluorescent reporter

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2-mOrange
   

   Use

   Lentiviral; Gateway destination vector

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Puro CAG IL13-CAR

   Plasmid

   #157742

   Purpose

   knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Chimeric antigen receptor targeting IL13
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 Ctnnb1.2

   Plasmid

   #59912

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(cd226)

   Plasmid

   #177900

   Purpose

   Bacterial expression of his10-tagged human cd226 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   CD226 (aa 288-336 only) (CD226 Human)
   

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCINeo-mCHMP5

   Plasmid

   #11771

   Depositor

   Sankar Ghosh

   Article

   Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

   Insert

   chromatin modifying protein 5 (Chmp5 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET22b-mEB3-mCherry-6xHis

   Plasmid

   #130985

   Purpose

   Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB3-mCherry-6xHis (Mapre3 Mouse)
   

   Tags

   mCherry-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-MED10

    Plasmid

    #15417

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED10 (Med10 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pLJM1 FLCN (R164A) FLAG GFP

    Plasmid

    #164980

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    R164A

    Promoter

    UBC

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FCK-Chronos-GFP

    Plasmid

    #80902

    Purpose

    Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304-MARK3_T211A

    Plasmid

    #107236

    Purpose

    Lentiviral gene expression vector for phospho-MARK3_T221A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYFP-ErbB2ΔC990

    Plasmid

    #66946

    Purpose

    Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C990. (see depositor comment below on…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Gal4-entry+NLS

    Plasmid

    #128013

    Purpose

    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4 DBD-SV40NLS-3x-Flag
    

    Use

    Gal4 entry vector with nls to generate tethering …

    Tags

    Gal4-DBD-SV40NLS-3xFlag

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYFP-ErbB2ΔC776

    Plasmid

    #66947

    Purpose

    Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C776 (see depositor comment below on …

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLJM1 FLCN (F118D) FLAG GFP

    Plasmid

    #164979

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    F118D

    Promoter

    UBC

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-MED27

    Plasmid

    #15424

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED27 (MED27 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. FLAG-MED21

    Plasmid

    #15422

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED21 (MED21 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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