We narrowed to 6,523 results for: sas

1. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Expression

   Bacterial

   Promoter

   Arabinose-inducible pBAD

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet1_His-TEV-EGFP

   Plasmid

   #223723

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   EGFP, not monomeric (has A207)
   

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2/CMV-Luc/PGK-SB100X

   Plasmid

   #192869

   Purpose

   Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

   Depositor

   Maria Castro, David Largaespada

   Article

   Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

   Inserts

   luc
   SB100X
   

   Expression

   Mammalian

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1749 (pDonor, R*MmeI)

   Plasmid

   #160733

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA
   

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

   Plasmid

   #136262

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2355_Tier3(PB)-iRFP_PuroR

   Plasmid

   #169654

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

    Plasmid

    #223287

    Purpose

    A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
    

    Use

    Synthetic Biology; Piggybac transposase insertabl…

    Expression

    Mammalian

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-p62-Silent_Mutation_WT

    Plasmid

    #187986

    Purpose

    human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation for resistance to sip62 #5

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid

    #164255

    Purpose

    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TRF2
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deletion of amino acids-86 and 496-542

    Promoter

    Tetracycline inducible promotor

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Duet1-His-TEV-mCherry-p62

    Plasmid

    #199780

    Purpose

    Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    6x His, TEV, mCherry

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid

    #169638

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-FKBP-GFP-SINTBAD

    Plasmid

    #216840

    Purpose

    Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FKBP-GFP-OPTN (2-119)

    Plasmid

    #208867

    Purpose

    Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    OPTN (OPTN Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    aa 2-119 only

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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