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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a Promoter

Use

Synthetic Biology

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB1_v1.3

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB3_v1.2

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

WPRE terminator

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and p53(1-85)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

zCas9

Use

CRISPR

Mutation

Zea mays codon-optimized Cas9

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - P381L in pENTR

Plasmid

#216570

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

P381L

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - D424N in pENTR

Plasmid

#216571

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

D424N

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - S411R in pENTR

Plasmid

#216603

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

S411R

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - P388S in pENTR

Plasmid

#216602

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P388S

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - P376S in pENTR

Plasmid

#216601

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P376S

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - E282G in pENTR

Plasmid

#216600

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

E282G

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - P217L in pENTR

Plasmid

#216599

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P217L

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - A202G in pENTR

Plasmid

#216598

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

A202G

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - G195S in pENTR

Plasmid

#216597

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

G195S

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - P152L in pENTR

Plasmid

#216596

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P152L

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - P152R in pENTR

Plasmid

#216595

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

P152R

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MECP2 - R91W in pENTR

Plasmid

#216593

Purpose

gateway cloning donor vector containing MECP2 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

MECP2 (MECP2 Human)

Use

Gateway entry plasmid

Mutation

R91W

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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