We narrowed to 8,454 results for: Ott

1. rag2:CPM_CE_pISceI

   Plasmid

   #223036

   Purpose

   Express CPM gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

   Insert

   CPM (CPM Human)
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. rag2:AGAP2_CE_pISceI

   Plasmid

   #223024

   Purpose

   Express AGAP2 gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

   Insert

   AGAP2 (AGAP2 Human)
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. rag2:CDK4_CE_pISceI

   Plasmid

   #223026

   Purpose

   Express CDK4 gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

   Plasmid

   #231237

   Purpose

   MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NES fused to the DNA-binding-domain of MYC (resid…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

   Plasmid

   #231235

   Purpose

   MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TAD deleted (delta1-143), DBD deleted (delta340-4…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

   Plasmid

   #231236

   Purpose

   MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLS fused to the DNA-binding-domain of MYC (resid…

   Promoter

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eSpCas9-hGeminin

   Plasmid

   #199344

   Purpose

   modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

   Depositor

   Iain Hagan

   Article

   Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

   Insert

   ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
   

   Use

   Tags

   cas9 c-term fused to hgemenin 1-110 fragment

   Expression

   Mammalian

   Mutation

   cas9 c-term fused to hgemenin 1-110 fragment

   Promoter

   cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

   Plasmid

   #208293

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

   Plasmid

   #208290

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpCas9(D10…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid

    #208291

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpRY(D10A/…

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

    Plasmid

    #194016

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.2)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmb and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pInducer 21-3xFlag-hMEFV-ΔPLD

    Plasmid

    #195479

    Purpose

    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain) with a 3xFlag tag at the N-terminus

    Depositor

    Thomas Henry

    Article

    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    deletion of residues 88 - 310 (the resulting pyri…

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307_CR-PDHA2S293A

    Plasmid

    #115204

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307_CR-PDHA2S291A/S293A

    Plasmid

    #115205

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid

    #115187

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid

    #115188

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid

    #115189

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid

    #115190

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid

    #115191

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Expression

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge