59,541 results

1. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

   Plasmid

   #214994

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Cyclin D1

   Plasmid

   #215050

   Purpose

   Expresses EGFP-Cyclin D1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF3036

   Plasmid

   #144512

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mScarlet-MYO10

   Plasmid

   #145179

   Purpose

   Expresses mScarlet-MYO10 in mammalian cells

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   mScarlet-MYO10 (MYO10 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-Lrrc59

   Plasmid

   #226564

   Purpose

   Encodes Lrrc59 protein labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF RBP-FL P53

   Plasmid

   #174042

   Purpose

   Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

   Depositor

   Stewart Loh

   Article

   Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

   Insert

   P53 gene (TP53 Human)
   

   Tags

   Thermoanaerobacter Tencongenesis ribose binding p…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. IKK-2 WT

   Plasmid

   #11103

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(Hygro) TagBFP-hNucleolin

   Plasmid

   #182592

   Purpose

   expresses bfp-hNucleolin in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hNucleolin (NCL Human)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn FLEx-FRT hM4Di-mCherry

    Plasmid

    #161576

    Purpose

    Expression of hM4Di-mCherry in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-FOXM1-shRNA-81

    Plasmid

    #89495

    Purpose

    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid

    #105287

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 20-108

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-Synaptojanin 1-170

    Plasmid

    #22292

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    K334R compared to Genbank ID NM_003895

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #195542

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EIF2AK2 gRNA (BRDN0001146342)

    Plasmid

    #75637

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV-CFP

    Plasmid

    #25998

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-CFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-N1-cofilin

    Plasmid

    #186750

    Purpose

    Expresses wildtype mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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