We narrowed to 19,107 results for: Tec
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pVH617
Plasmid#169606PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
RS17060_pMQ30_KO_Kan
Plasmid#185396PurposeNon-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11DepositorInsertsRR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region
Available SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS17055_pMQ30_KO_Kan
Plasmid#185398PurposeNon-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11DepositorInsertsRR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region
Available SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox1
Plasmid#50778Purposeretroviral expression of mouse Sox1DepositorAvailable SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterU6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGWB527
Plasmid#74869PurposeGateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GATA1
Plasmid#67068PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Clathrin-19
Plasmid#56584PurposeLocalization: Clathrin Vesicles, Excitation: 513, Emission: 527DepositorAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGWB525
Plasmid#74867PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3020(gRNA X-2)
Plasmid#73282PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TNF
Plasmid#67062PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Reg-L-IFP-F[2]+Venus (mammalian)
Plasmid#52903PurposeBinary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertReg-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
Plasmid#52905PurposeBinary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertCat-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only
