We narrowed to 24,446 results for: lars

1. SHLD3 N-terminal sgRNA

   Plasmid

   #207094

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTACTGCAGAATGACTACAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NBS1 C-terminal sgRNA

   Plasmid

   #207092

   Purpose

   pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TAAAAAGGAGAAGATAACTG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RNF169 C-terminal sgRNA

   Plasmid

   #207099

   Purpose

   pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ACACTTCATTAGGTGCTACT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SHLD1 N-terminal sgRNA

   Plasmid

   #207101

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATGGCAGGACTATGGCAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ATM C-terminal sgRNA

   Plasmid

   #207097

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTTCTAAAGGCTGAATGAAA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

   Plasmid

   #218654

   Purpose

   Knockout vector for mouse Fam160b1 (Fhip2A)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Fam160b1 (Fhip2a Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

   Plasmid

   #218640

   Purpose

   Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   delPYD (I125M-END)

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221 hNLRP3 R262W (disease-associated mutation)

   Plasmid

   #218647

   Purpose

   Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   R262W

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR221 msNLRP3(ΔPYD, I125M-END)

   Plasmid

   #218641

   Purpose

   Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   delPYD (I125M-END)

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

    Plasmid

    #218655

    Purpose

    Knockout vector for mouse Fam160b1 (Fhip2A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    Fam160b1 (Fhip2a Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MGS0156_pET29b

    Plasmid

    #215410

    Purpose

    Expression construct for MGS0156 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    MGS0156
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SHLD3 KO sgRNA #1

    Plasmid

    #207105

    Purpose

    pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CGCTATCAAGATTTATACCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgRNA TR KO 2

    Plasmid

    #207610

    Purpose

    sgRNA 2 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    TCAGGCCGCAGGAAGAGGAA
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

    Plasmid

    #215000

    Purpose

    Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-PunyEEER

    Plasmid

    #208322

    Purpose

    Bacterial expression of the His-tagged peptide PunyEEER.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    PunyERRE
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMMK201

    Plasmid

    #207131

    Purpose

    Type 2 ptac promoter part

    Depositor

    Nicholas Shikuma

    Article

    Alker et al mBio. 2023 Aug 2:e0150223. doi: 10.1128/

    Insert

    ptac promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPOM042_P15678_MultiGeneBackbone

    Plasmid

    #216467

    Purpose

    Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPOM041_P15678_SingleGeneBackbone

    Plasmid

    #216466

    Purpose

    Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPOM032_P4_tSynth30

    Plasmid

    #216457

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth30
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPOM031_P4_tSynth29

    Plasmid

    #216456

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth29
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPOM030_P4_tSynth27

    Plasmid

    #216455

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth27
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPOM029_P4_tSynth25

    Plasmid

    #216454

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth25
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPOM028_P4_tSynth3

    Plasmid

    #216453

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator synth3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPOM027_P4_tSynthGuo

    Plasmid

    #216452

    Purpose

    Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Terminator guo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPOM025_P2_pTUP11

    Plasmid

    #216450

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter tup11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPOM023_P2_pAPL4

    Plasmid

    #216448

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter apl4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPOM021_P2_pTUB1

    Plasmid

    #216446

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter tub1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HaloTag KO sgRNA

    Plasmid

    #207102

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTCGATGTTGGTCCGCGCGA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge