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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13b (Kif13b Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ChRmine-mRuby2-ST

Plasmid

#227777

Purpose

Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChRmine-mRuby2-ST

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDT-sgRNA-XMAS-1x

Plasmid

#164413

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Dlx-FLEX-hM4Di-mCherry

Plasmid

#209701

Purpose

Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

hM4D(Gi)

Use

AAV

Tags

mCherry

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP4

Plasmid

#42313

Depositor

Thierry Galli

Article

Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

Insert

VAMP 4 (VAMP4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pGFP-LacI

Plasmid

#179510

Purpose

Expresses EGFP-LacI in mammalian cells

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

LacI

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-eNpHR-EYFP

Plasmid

#89876

Purpose

To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

eNpHR=EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

TetO

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-CANTHipi

Plasmid

#53301

Purpose

Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtO

Use

Low copy number bacterial cloning vector

Tags

6His

Promoter

Trc

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon

Plasmid

#28022

Depositor

Qing Zhong

Article

Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

Insert

Rubicon (RUBCN Human)

Tags

3xFlag and EGFP

Expression

Mammalian

Available Since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC-nSyb-IVS-PhiC31

Plasmid

#133868

Purpose

Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-HIS3

Plasmid

#229230

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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