We narrowed to 12,134 results for: cel.2

1. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

   Plasmid

   #200123

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP‐RNASEH2C

   Plasmid

   #108698

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2C (RNASEH2C Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRRL-5xTetO-pEF-H2B-Citrine-SV40

   Plasmid

   #186966

   Purpose

   Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   5xTetO-pEF-H2B-Citrine-SV40
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   5xTetO-pEF-1alpha

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaM34(D93A/D129A)/pIRES2-eGFP

   Plasmid

   #111517

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Expression

   Mammalian

   Mutation

   D93A/D129A

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaM12(D20A/D56A)/pIRES2-eGFP

   Plasmid

   #111512

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   calmodulin [Xenopus laevis] (calm2 Frog)
   

   Expression

   Mammalian

   Mutation

   D20A/D56A

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209016

   Purpose

   Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

   Plasmid

   #200124

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

    Plasmid

    #200125

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-TdIF2

    Plasmid

    #199699

    Purpose

    visualization of nucleolus in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    TdIF2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEAST_J23101-bphD

    Plasmid

    #128138

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-tTRE-scFv-SunTag

    Plasmid

    #193138

    Purpose

    Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    scFv-SunTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Daxx/pEGFP

    Plasmid

    #119021

    Purpose

    Express GFP-Daxx in mamalian cells

    Depositor

    Xiaolu Yang

    Article

    Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

    Insert

    death-domain associated protein (Daxx) (DAXX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. YPET-GGA3

    Plasmid

    #18841

    Depositor

    Martin Schwartz

    Article

    Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

    Insert

    YPET-GGA3 (GGA3 Human)
    

    Tags

    His6 and myc

    Expression

    Mammalian

    Mutation

    The fluorescent protein YPET was added to the ami…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)_UGControl

    Plasmid

    #133875

    Purpose

    Expresses UGControl in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Tags

    flexible linker - mEGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_UG3

    Plasmid

    #133874

    Purpose

    Expresses UG3 in mammalian cells.

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Tags

    helical linker - mEGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1-EGFP-Vasa open (K295N) (RR228)

    Plasmid

    #206435

    Purpose

    Vasa expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Vasa (vas Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Mutation

    K282N

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-syn-SnFR-gamma2-minWPRE

    Plasmid

    #165497

    Purpose

    AAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisation

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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