We narrowed to 58,550 results for: tra

1. pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag

   Plasmid

   #209660

   Purpose

   Expression of iATPSnFR2, medium affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95K.HaloTag
   

   Use

   AAV

   Mutation

   A95K

   Promoter

   GFAP

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSK267

   Plasmid

   #129272

   Purpose

   "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   NLS-VP16-ZF 43-8-NES
   

   Tags

   6xHA

   Expression

   Yeast

   Promoter

   SUP35

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SFG.CNa12_opt.IRES.eGFP

   Plasmid

   #22490

   Depositor

   Martin Pule

   Article

   Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

   Insert

   Codon optimised Calcineurin A mutant 12 (PPP3CA Human)
   

   Use

   Retroviral

   Mutation

   T351E; L354A

   Available Since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-mTAF12

   Plasmid

   #44342

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF12 (Taf12 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-V5-Sec61B

   Plasmid

   #120244

   Purpose

   Expresses V5-tagged Sec61B in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Sec61B (Sec61b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

   Plasmid

   #170466

   Purpose

   The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Bakhache et al bioRxiv 2021

   Insert

   Nucleocapsid
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPYngK+-hp

   Plasmid

   #48121

   Purpose

   Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   codon optimized signal peptide of YngK
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

   Plasmid

   #159847

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-HsSENP3(307-574)

   Plasmid

   #71481

   Purpose

   Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-306 (catalytic domain only)

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-WRAP53beta

    Plasmid

    #64676

    Purpose

    expresses Wild type WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. RNAseq_cal_Tuschl

    Plasmid

    #59426

    Purpose

    Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

    Depositor

    Thomas Tuschl

    Article

    Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

    Insert

    RNAseq_cal_Tuschl
    

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-nTR

    Plasmid

    #21812

    Depositor

    Wafik El-Deiry

    Article

    Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

    Insert

    pQE-nTR (TNFSF10 Human)
    

    Expression

    Bacterial

    Mutation

    His tag and aa95-113 were removed from pQE-hTR

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

    Plasmid

    #159846

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TXNIP-py

    Plasmid

    #200090

    Purpose

    Dox-inducible human TXNIP with py motif mutations (ppcy,ppty -->paca,pata) in pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    TXNIP (TXNIP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    py motifs mutated (aa331-334 and 375-378)

    Promoter

    CMV

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWB-Flag-IKKe

    Plasmid

    #44115

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    IKKe (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available Since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTP130 (pTA106_TnsABC(Tn6900))

    Plasmid

    #164261

    Purpose

    Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
    

    Expression

    Bacterial

    Mutation

    (tnsA) Changed Alanine 125 to Aspartic acid

    Promoter

    Lactose

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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