We narrowed to 81,846 results for: TRI

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pcDNA3-Venus-cpVenus-FLARE-CKAR

Plasmid

#123343

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 GST MetaP2

Plasmid

#112750

Purpose

express HA-GST-FRB-MetaP2

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

HA-GST-FRB-MetaP2 (METAP2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPN186

Plasmid

#91573

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN008

Plasmid

#91576

Purpose

Express sgRNA targeting human BCL11A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BCL11A (BCL11A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA-H1.4CTD

Plasmid

#157802

Purpose

Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MMP8 (P78S)

Plasmid

#29546

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

P78S

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
Sumo3 PolQM1 deltains3

Plasmid

#78459

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2497-2529

Promoter

T7

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pPN187

Plasmid

#91574

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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His-GINA3

Plasmid

#74980

Purpose

Expresses His tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )

Tags

His

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pPN094

Plasmid

#91621

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag GFP 4E-BP1 Omp25

Plasmid

#112760

Purpose

express GFP-4EBP1-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)

Use

Lentiviral

Tags

Flag eGFP and Omp25

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP EMI1

Plasmid

#158947

Purpose

Express human EMI1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

EMI1 (FBXO5 Human)

Tags

2X FLAG 2X STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pCMV

Plasmid

#8815

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) HIS-tag pET new RBS

Plasmid

#8525

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains improved Ribosomal binding site; makes …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-NHL only-LIN-41

Plasmid

#52723

Purpose

retroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

NHL-only contains only an initiating methionine a…

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-7CtoA-LIN-41

Plasmid

#52726

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12, 15, 61, 66, 69, 91, and 94 …

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA_P32T

Plasmid

#60825

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Article

Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d

Insert

ITPA (ITPA Human)

Tags

His

Expression

Bacterial

Mutation

P32T

Promoter

T7

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only