We narrowed to 74,949 results for: Rest

1. pJK580

   Plasmid

   #71694

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. XE182 rat Frizzled-1-HA-pCD

   Plasmid

   #16818

   Depositor

   Randall Moon

   Insert

   frizzled-1 (Fzd1 Rat)
   

   Use

   Xenopus expression

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pLIX403-EYFP-ccdB-EBFP2

   Plasmid

   #158553

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLS1/-11

   Plasmid

   #31492

   Depositor

   Kathleen Matthews

   Insert

   Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pRL-TK 4x wt

   Plasmid

   #11313

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-S-P1-FLAG

   Plasmid

   #177095

   Purpose

   P1 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein P1 (S SARS-CoV-2)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. Paxillin_PSCFP2_ mEOS2

   Plasmid

   #114192

   Purpose

   studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling

   Depositor

   Aleksandra Radenovic

   Insert

   Paxillin (PXN Chicken)
   

   Use

   Tags

   PS-CFP2 and mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. XE126 Rfz1myc CS2+

   Plasmid

   #16798

   Depositor

   Randall Moon

   Insert

   Rfz1 (Fzd1 Rat)
   

   Use

   Xenopus expression

   Tags

   myc

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pENTRY4_ Pi21

   Plasmid

   #126904

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHXPL

    Plasmid

    #17667

    Depositor

    Cecilia Lo

    Insert

    Cx43 promoter (Gja1 Mouse)
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UBC45

    Plasmid

    #25469

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc45.001.200
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pGEX-5X-1 SmB-N

    Plasmid

    #129075

    Purpose

    generation of GST-tagged N-terminal part of SmB protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    SmB (SNRPB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains amino acids 1-492

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTLPflaG-small

    Plasmid

    #45476

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. HC080

    Plasmid

    #59545

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag

    Depositor

    Daniel Klionsky

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5 TBRI K to R

    Plasmid

    #19159

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta 1 (TGFBR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K420R mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCMV6-XL4 FLAG-TM9SF4-Fc

    Plasmid

    #115770

    Purpose

    Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    TM9SF4 (TM9SF4 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PP-UEV1A

    Plasmid

    #25111

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0255c:C34858
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pGPDluxAB(Ura)

    Plasmid

    #1119

    Depositor

    Susan Lindquist

    Article

    Singer et al Mol Cell 1998 Apr;1(5):639-48.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pNC891

    Plasmid

    #20397

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    YFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=M (see schematic)

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Abr in pAcG2T

    Plasmid

    #38162

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

23. U6-Lin28aIR

    Plasmid

    #120530

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK GFP Vinculin

    Plasmid

    #50531

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pBac[6xP3-Tc-hsp-DsRedExpress-SV40]

    Plasmid

    #86448

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Drives dsRedExpress (red fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    6xP3-Tc-hsp-DsRedExpress-SV40
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPS2971

    Plasmid

    #8926

    Depositor

    Pamela Silver

    Article

    Devroe et al BMC Biotechnol. 2002 Aug 28. 2():15.

    Insert

    NDR1 (STK38 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

27. RS314

    Plasmid

    #17542

    Depositor

    Bert Vogelstein

    Article

    Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pRSII419

    Plasmid

    #35460

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast centromere vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV6-XL4 FLAG-TMEFF2-Fc

    Plasmid

    #115774

    Purpose

    Expression of the extracellular domain of TMEFF2 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    TMEFF2 (TMEFF2 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 830 pcDNA3 GFP PTEN 1-336

    Plasmid

    #10927

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-336

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only