We narrowed to 8,596 results for: Set

1. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ES-FUCCI

   Plasmid

   #62451

   Purpose

   SMX_DEST vector with 3 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
   minPGK::Cherry-hCdt1(30-120)-bGHpA
   PGK::HygroR-bGpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIV643

   Plasmid

   #226182

   Purpose

   Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   uidA marker flanked with targeting regions to KU70 gene in K.phaffii
   

   Expression

   Bacterial

   Promoter

   TEF1p

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBC-MT2T3

   Plasmid

   #50594

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, TaU3t plus, U6-26p, T3
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-SisTnpB1–ωRNA

    Plasmid

    #228365

    Purpose

    Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

    Depositor

    Tao Liu

    Article

    Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

    Insert

    SisTnpB1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-13Myc-kanMX6

    Plasmid

    #39294

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1 LAMP1-mNeonGreen HRD

    Plasmid

    #207549

    Purpose

    Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible LAMP1-mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PECAM1-mRuby3-T2A-secNluc

    Plasmid

    #235607

    Purpose

    Donor plasmid for knock-in of mRuby3-T2A-secNluc into the human PECAM1 locus

    Depositor

    Kai Wang

    Article

    Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

    Insert

    PECAM1 homologous recombination arms with PuroR drug selection cassette
    

    Use

    CRISPR

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Tags

    no tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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