We narrowed to 58,550 results for: tra
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Plasmid#24363DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV Aquamarine-shadowY tandem
Plasmid#157775PurposeProduces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)DepositorTypeEmpty backboneTagsAquamarine and shadowYExpressionMammalianAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mTAZ-WW (86)
Plasmid#40915DepositorInsertTAZ (Wwtr1 Mouse)
TagsGSTExpressionBacterialMutationContains mTAZ 14-3-3 binding site and WW domain (…PromoterTacAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54
Plasmid#172451PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tagDepositorAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ914
Plasmid#44489DepositorInsertMinimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
UseGateway expression cloneAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 L182A/D183A/I184A/G185A
Plasmid#67608Purposebacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
TagsGSTExpressionBacterialMutationcatalytic mutant LDIG(182-185)AAAAPromotertacAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH028
Plasmid#80393PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)DepositorUseSynthetic BiologyTagsFused by GS linkers to 4 SH3 domainsExpressionBacterialMutationGlycine 448 changed to Alanine, makes it primaril…Available SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159851PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-Cx43
Plasmid#69442Purposemammalian expression of rat Cx43DepositorInsertCx43 (Gja1 Rat)
ExpressionMammalianAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47451PurposeAAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NG_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47448PurposeAAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NI_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47449PurposeAAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NN_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47447PurposeAAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NG_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH734-CEN-minHIS3
Plasmid#38227DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH737-CEN-maxHIS3
Plasmid#38229DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3 (=GPD)Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
TagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only