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Showing: 6301 - 6320 of 59495 results
  1. pSPYngK-hp

    Plasmid
    #48118
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter
    Depositor
    Insert
    signal peptide of YngK
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. pTC-CMV-Tet-YAP

    Plasmid
    #85579
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liver
    Depositor
    Inserts
    CreER
    YAP
    Use
    Cre/Lox
    Tags
    Flag (2x)
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47450
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Insert
    TALE BB(N136_0.5HD_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. IpO

    Plasmid
    #48233
    Purpose
    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
    Depositor
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    URA3 promoter and none
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pLY239

    Plasmid
    #184152
    Purpose
    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Insert
    s-tracrRNA-R3
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    araBAD promoter
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pOPO636

    Plasmid
    #195299
    Purpose
    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).
    Depositor
    Insert
    TnsABCQ operon of McCAST (Tn7575)
    Expression
    Bacterial
    Promoter
    IPTG inducible lac promoter
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 6301 - 6320 of 59495 results