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Showing: 6341 - 6360 of 176472 results

  1. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Publication
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128605-plasmid-map-sequence-id-318004

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  6. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100903-plasmid-map-sequence-id-196607

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  10. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    91854-plasmid-map-sequence-id-176529

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  11. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    87836-plasmid-map-sequence-id-176310

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  16. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    80811-plasmid-map-sequence-id-152800

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  17. Talin-CS

    Plasmid
    #83377
    Purpose
    Control FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Publication
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted at C-terminal o…
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83377-plasmid-map-sequence-id-158762

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Showing: 6341 - 6360 of 176472 results