159,589 results

1. pILGFPB5A

   Plasmid

   #83556

   Purpose

   Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   GAL1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p212 pCMV-EGFP/RFP

   Plasmid

   #8389

   Purpose

   Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. BSA1_His

   Plasmid

   #202766

   Purpose

   bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   BSA1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HaloTag-bActinCDS-bActinUTR-MS2V5

   Plasmid

   #102718

   Purpose

   Halo-Actin reporter for detection of both mRNA and protein

   Depositor

   Robert Singer

   Article

   Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   HaloTag-bActinCDS-bActin3'UTR-MS2V5
   

   Use

   Lentiviral

   Tags

   HaloTag and MS2V5

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-M4-AR-S81A

   Plasmid

   #171241

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser81Ala (AR-S81A)

   Promoter

   CMV

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. plentiCMV-sp-HPV-TM(ICAM)

   Plasmid

   #196510

   Purpose

   Express HPV antigen epitope with ICAM transmembrane domain

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   a HPV epitope with CD8a stalk linker and ICAM TM domain
   

   Use

   Lentiviral

   Tags

   3xflag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-HsSENP2

   Plasmid

   #71466

   Purpose

   Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 2 (SENP2 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCAG:H2B-EGFP

   Plasmid

   #32599

   Depositor

   Virginia Papaioannou, Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

   Insert

   H2B-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCL-CTIG

    Plasmid

    #14901

    Purpose

    3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

    Depositor

    Inder Verma

    Article

    Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

    Insert

    CMV-tTA-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC1-SypHer3s-dmito

    Plasmid

    #108119

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-dmito
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-FGFR2

    Plasmid

    #116741

    Purpose

    Lentiviral expression of FGFR2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR2 (FGFR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29b-IPP1-His

    Plasmid

    #124137

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Inorganic pyrophosphatase (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. KAT14-pDEST10

    Plasmid

    #118382

    Purpose

    HIS-tagged insect cell expression vector with KAT14.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    KAT14 (KAT14 Human)
    

    Use

    Tags

    HIS

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMVTRE3G eGFP Neo (w821-1)

    Plasmid

    #27569

    Depositor

    Eric Campeau

    Insert

    Enhance Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMVtight eGFP Blast (w782-1)

    Plasmid

    #26584

    Purpose

    2nd generation lentiviral vector

    Depositor

    Eric Campeau

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral; Tet-on advanced vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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