We narrowed to 76,945 results for: Rest

1. pFA6a-link-ymBaojin-SpHis5

   Plasmid

   #224082

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with SpHis5 selection

   Depositor

   Bas Teusink

   Insert

   ymBaoJin
   

   Tags

   ymBaoJin

   Expression

   Yeast

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

   Plasmid

   #86453

   Purpose

   UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Syn-FLEX-MCS-WPRE-SV40 - GCH8

   Plasmid

   #230818

   Purpose

   AAV cloning vector for CRE-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TMC1-TwinStrepII

   Plasmid

   #83267

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   TMC1 (Tmc1 Mouse)
   

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2T-Rac1-wt

   Plasmid

   #12977

   Depositor

   Gary Bokoch

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-Flag-Siah2 Ring Mutant

   Plasmid

   #122042

   Purpose

   express Siah2 RM

   Depositor

   Ze'ev Ronai

   Insert

   Siah2 RM (Siah2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ring mutant deletion H98, L99A, V100G, C101A

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-XL4 FLAG-NGRN-Fc

   Plasmid

   #115773

   Purpose

   Expression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.

   Depositor

   Davide Comoletti

   Insert

   NGRN (NGRN Human)
   

   Tags

   FLAG and hIgG/Fc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

   Plasmid

   #183252

   Purpose

   Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

   Depositor

   Alexander McLellan

   Insert

   anti-CD19 scFvFc (FMC-63)
   

   Expression

   Mammalian

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRZ

   Plasmid

   #27663

   Depositor

   Graeme Conn

   Article

   Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Type

   Empty backbone

   Use

   Rna transcription

   Tags

   HDV Ribozyme

   Available Since

   April 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-T4gene30

    Plasmid

    #165554

    Purpose

    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T4-DNA Ligase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET His6 MBP prescission LIC cloning vector (HMPKS)

    Plasmid

    #29721

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, MBP, and Prescission cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-flag-UBR2

    Plasmid

    #50573

    Purpose

    Expresses FLAG-tagged mouse UBR2 in mammalian cells.

    Depositor

    Alexander Varshavsky

    Insert

    UBR2 (Ubr2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTCF7 HLA-B*07:02

    Plasmid

    #180446

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*07:02
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. hTLR5 flag

    Plasmid

    #13088

    Depositor

    Ruslan Medzhitov

    Insert

    TLR5 (TLR5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced by a mammalia…

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. hTLR3 flag

    Plasmid

    #13084

    Depositor

    Ruslan Medzhitov

    Insert

    TLR3 (TLR3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-17 have been replaced by mammalian …

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-U6

    Plasmid

    #19799

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pLinker-6xHA-HXGPRT-LoxP

    Plasmid

    #86552

    Purpose

    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

    Depositor

    David Sibley

    Insert

    Linker-6xHA-HGPRT-LoxP
    

    Use

    Expression in toxoplasma gondii

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-V5-SBP-C1-HsPTB1

    Plasmid

    #64925

    Purpose

    Expresses V5- and SBP-tagged PTB1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    PTB1 (PTBP1 Human)
    

    Tags

    V5 and SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCEP4YPet-MAMM

    Plasmid

    #14032

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    YPet
    

    Expression

    Mammalian

    Mutation

    Mammalian optimized YPet

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid

    #72259

    Purpose

    Express FLPe and tdTomato under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. tet-pLKO.puro_shLuc

    Plasmid

    #110422

    Purpose

    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBS302

    Plasmid

    #11925

    Depositor

    Brian Sauer

    Article

    Sauer et al Methods Enzymol. 1993;225:890-900. doi:

    Insert

    STOP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBABE.is4-mOpn

    Plasmid

    #74410

    Purpose

    Expresses mouse Osteopontin from transcript tv4 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    mouse Opn (Spp1 Mouse)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    MMLV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-ps-CFP

    Plasmid

    #13034

    Purpose

    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence

    Depositor

    Doug Golenbock

    Insert

    photo-switchable cyan fluorescent protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. PCDH-BCAT2

    Plasmid

    #196204

    Purpose

    overexpress BCAT2

    Depositor

    Jianjun Chen

    Insert

    BCAT2 (BCAT2 Human)
    

    Use

    Lentiviral

    Mutation

    NO

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCIG3 Rab5CA

    Plasmid

    #78268

    Purpose

    Rab5 Constitutively active (Q79L) expression

    Depositor

    Felicia Goodrum

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    CMV, Lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Tags

    ymScarletI

    Expression

    Yeast

    Available Since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pCMVHA-E2F5

    Plasmid

    #24213

    Purpose

    Mammalian expression of human E2F5 with HA tag

    Depositor

    Kristian Helin

    Insert

    E2F5 (E2F5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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