We narrowed to 11,748 results for: pcdna

1. pcDNA3-LifeAct-FRB-DMVF-181-N

   Plasmid

   #87610

   Purpose

   FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-FRB-DMVF181-N
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

   Plasmid

   #138197

   Purpose

   FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

   Plasmid

   #138199

   Purpose

   FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

   Plasmid

   #138200

   Purpose

   FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains leucine-to-tryptophan substitution at re…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-mCherry-PKCsub-RLuc8N-NES

   Plasmid

   #138213

   Purpose

   Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

   Plasmid

   #138299

   Purpose

   Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKCsub(T/A)-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

   Plasmid

   #111454

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-492), C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG-RNMT 121-476

   Plasmid

   #112707

   Purpose

   Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 2-120

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

   Plasmid

   #169273

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 WT minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

    Plasmid

    #169274

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 GT>A minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NM_018077.2:c.(541+1_541+2delinsA)

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

    Plasmid

    #187582

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF7 (SRSF7 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

    Plasmid

    #187576

    Purpose

    Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    LMNA (LMNA Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

    Plasmid

    #187575

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

    Plasmid

    #187585

    Purpose

    Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NPAT (NPAT Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

    Plasmid

    #187584

    Purpose

    Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SP100 (SP100 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

    Plasmid

    #187587

    Purpose

    Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SMN2 (SMN2 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

    Plasmid

    #187579

    Purpose

    Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PSPC1 (PSPC1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

    Plasmid

    #187580

    Purpose

    Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge