We narrowed to 2,364 results for: NIG

1. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

   Plasmid

   #176484

   Purpose

   TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac-TetO-En1-t2a-Foxa2-hygro

   Plasmid

   #176483

   Purpose

   TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MADR pDonor smFP-HA (dark) WPRE

   Plasmid

   #131007

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-HA WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   HA - 10 total HA epitope tages

   Expression

   Mammalian

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor smFP-V5 (dark) WPRE

   Plasmid

   #131006

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   V5 - 10 total of V5 epitope tag

   Expression

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor smFP-Myc (dark) WPRE

   Plasmid

   #130987

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-Myc WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   Myc - 10 total Myc tags

   Expression

   Mutation

   "dark" variant with GGG fluorophore ver…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid

    #131005

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-FLAG WPRE
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    FLAG - 10 total FLAG tags

    Expression

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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