We narrowed to 4,504 results for: pcr

1. Phleo/BB2

   Plasmid

   #1808

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   Phleo/BB2
   

   Use

   Tags

   BB2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

2. Phleo/V5

   Plasmid

   #1809

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   Phleo/V5
   

   Use

   Tags

   V5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. neo/BB2

   Plasmid

   #1810

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   Neo/BB2
   

   Use

   Tags

   BB2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

4. BB2/neo

   Plasmid

   #1811

   Depositor

   Christian Tschudi

   Article

   Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

   Insert

   BB2/neo
   

   Use

   Tags

   BB2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pJW1310

   Plasmid

   #61253

   Purpose

   Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   U6 promoter
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-TRP1

   Plasmid

   #41595

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJW1311

   Plasmid

   #61254

   Purpose

   Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E) targeting Y61A9L9.1
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-pgk-DIO-rvCRY-WPRE

   Plasmid

   #182060

   Purpose

   Plasmids for production of AAV-based TFactivity reporter

   Depositor

   Kentaro Abe

   Article

   Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hu Pgk1

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAH4

    Plasmid

    #41828

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-3HA-TRP1

    Plasmid

    #41599

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-13Myc-TRP1

    Plasmid

    #41601

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    13Myc and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-Neq2X

    Plasmid

    #182365

    Purpose

    Expresses Neq2X DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDB4282

    Plasmid

    #98701

    Purpose

    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB4283

    Plasmid

    #98702

    Purpose

    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAH5

    Plasmid

    #41829

    Purpose

    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7,TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAH6

    Plasmid

    #41830

    Purpose

    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7,TEF

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-GST-TRP1

    Plasmid

    #41603

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAH2

    Plasmid

    #41826

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAP1836

    Plasmid

    #137087

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-TRP1-PGAL1

    Plasmid

    #41606

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    TRP1 reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAP1837

    Plasmid

    #137086

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAP3041

    Plasmid

    #137083

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    GFP11::3xFlag::tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAP1680

    Plasmid

    #137088

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAH3

    Plasmid

    #41827

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AbVec1.1-IGKC

    Plasmid

    #80796

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-3HA-His3MX6

    Plasmid

    #41600

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge