We narrowed to 1,409 results for: 4-Oct

1. pcmv3-IRG1-L272Q-HA

   Plasmid

   #198184

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The leucine in IRG1 at position 272 mutates to gl…

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcmv3-STING-C88S-FLAG

   Plasmid

   #198187

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C88S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcmv3-IRG1-H159Q-HA

   Plasmid

   #198183

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The histidine in IRG1 at position 159 mutates to …

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcmv3-IRG1-H277Y-HA

   Plasmid

   #198185

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The histidine in IRG1 at position 277 mutates to …

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNIC-ZB-SPRTNwt-FL

   Plasmid

   #110217

   Purpose

   Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pW34

   Plasmid

   #158210

   Purpose

   LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Insert

   LuxABCDE
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pW5Y_Apr

   Plasmid

   #158211

   Purpose

   BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

   Depositor

   Yasuo Yoshikuni

   Article

   Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNIC-ZB-SPRTN-E112A-FL

   Plasmid

   #110218

   Purpose

   Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   His6 and ZB

   Expression

   Bacterial

   Mutation

   E112A, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid

    #110219

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcmv3-STING-C1471S-FLAG

    Plasmid

    #198188

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C1471S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 147 mutates to …

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcmv3-STING-C64S,C65S-FLAG

    Plasmid

    #198186

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 64/65 mutates t…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS' and lambda(ts) and proK

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3.1-H-Luc_nucl

    Plasmid

    #113931

    Purpose

    Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_nucl
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3.1-H-Luc_cyto

    Plasmid

    #113929

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET51b(+)_H-Luc

    Plasmid

    #113924

    Purpose

    Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1-S-Luc_cyto

    Plasmid

    #113926

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KBB460

    Plasmid

    #185094

    Purpose

    NMA111-GFP wild type under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111-GFP

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1-H-Luc_mito

    Plasmid

    #113930

    Purpose

    Plasmid encoding the mitochondrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_mito
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mFzd4

    Plasmid

    #42256

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pINTO-N-FH

    Plasmid

    #65924

    Purpose

    Inducible expression of proteins fused to an N-terminal Flag-HA tag (FH)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2 (T-REx)

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1-S-Luc_mito

    Plasmid

    #113927

    Purpose

    Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_mito
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUPD2 SV40-AcrIIA4 (GB3333)

    Plasmid

    #160605

    Purpose

    SV40-AcrIIA4 codon optimized for N.benthamiana

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3.1-S-Luc_nucl

    Plasmid

    #113928

    Purpose

    Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_nucl
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KBB439

    Plasmid

    #185093

    Purpose

    NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::NMA111-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KBB280

    Plasmid

    #185073

    Purpose

    Larger fusion of Nma111 nuclear localization signal to GFP

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111 aa 1-83

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KBB282

    Plasmid

    #185074

    Purpose

    Smaller fusion of Nma111 nuclear localization signal to GFP

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111 aa 1-35

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KBB465

    Plasmid

    #185098

    Purpose

    NMA111-GFP with NLS1 deleted under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111DNLS1-GFP

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KBB462

    Plasmid

    #185095

    Purpose

    NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::nma111Dnls1Dnls2-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. KBB466

    Plasmid

    #185099

    Purpose

    NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111DNLS1DNLS2-GFP

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. KBB467

    Plasmid

    #185100

    Purpose

    NMA111-GFP with NLS2 deleted under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111DNLS2-GFP

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCSC-NGN2-IRES-ISL1-T2A-LHX3

    Plasmid

    #221814

    Purpose

    Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

    Depositor

    Baojin Ding

    Article

    Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

    Inserts

    NEUROG2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid

    #154846

    Purpose

    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Long isoform) (STX5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCitrine

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CXCR4-SZ158a QTY

    Plasmid

    #162442

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-X-C chemokine receptor type 4 (CXCR4 Human)
    

    Use

    Tags

    his-tag, Rho-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CXCR4-SZ146a QTY

    Plasmid

    #162443

    Purpose

    Expression in E coli and perform ligand binding in solution

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-X-C chemokine receptor type 4 (CXCR4 Human)
    

    Use

    Tags

    his-tag, Rho-tag

    Expression

    Bacterial

    Mutation

    All L, I, V, F in the transmembrane region mutate…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CXCR4-SZ158a-sfGFP

    Plasmid

    #162446

    Purpose

    Expression in HEK293T cell and compete ligand singaling against full-length receptors

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-X-C chemokine receptor type 4 (CXCR4 Human)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    truncation from aa52 to aa257

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSLC-254

    Plasmid

    #124291

    Purpose

    Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

    Insert

    vsfGFP-0
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

    Plasmid

    #67294

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge