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Plasmid#71123PurposeExpression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coliDepositorInsertpantothenate kinase
UseTagsH6ExpressionBacterialMutationArg175 is mutated to a HisPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2lox-cAMPr R626A
Plasmid#102437PurposecAMPr with a point mutation R626A which theoretically decreases its affinity for cAMPDepositorInsertcAMPr
UseCre/LoxTagsExpressionMammalianMutationPKA-R R626APromoterAvailable sinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-4a
Plasmid#61314PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and TRX-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-trAMO
Plasmid#20112DepositorInsertp450 reductase and truncated amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCri-6a
Plasmid#61315PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsGST-tag and His-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-12b
Plasmid#61334PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-9a-Strep
Plasmid#61341PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsStrep-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046465
Plasmid#149112PurposeYeast Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCri-15b
Plasmid#61336PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionYeastMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-1a
Plasmid#60816PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and MBP-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-16a
Plasmid#61324PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-14a
Plasmid#61322PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and LSL-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-8a-Strep
Plasmid#61340PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSII-FRT-MCS
Plasmid#205988PurposeVector containing FRT and FRT-F3 sitesDepositorTypeEmpty backboneUsePcr vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T2_gRNA
Plasmid#199388PurposepDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNADepositorInsertnrg1 type II sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a- phage MazG-like/Atd1
Plasmid#195244PurposeExpersses phage MazG-like/Atd1DepositorInsertphage MazG
UseTagsFlag and TwinStrepExpressionBacterialMutationCatalytic site mutantPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Prp39-pET21b
Plasmid#111286PurposePrp39 expression plasmidDepositorInsertPRP39
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-Prp28
Plasmid#111285PurposePrp28 expression plasmidDepositorInsertPRP28
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Prp40-pET21b
Plasmid#111287PurposePrp40 expression plasmidDepositorInsertPRP40
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN20 (deltaL)
Plasmid#80112PurposeLate2(delta)-4GS-I3-01-myc-PHDepositorInsertEPN-20 (L1)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK227
Plasmid#71126PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsGyrA inteinExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK136
Plasmid#71122PurposeExpression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coliDepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCri-14b
Plasmid#61335PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and LSL-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-12a
Plasmid#61320PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-7a-Strep
Plasmid#61339PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsStrep-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-1a-Strep
Plasmid#61337PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and MBP-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-6b
Plasmid#61329PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsGST-tag and His-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-11a
Plasmid#61319PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IQGAP1
Plasmid#30112DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagstandem EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPlexAtetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-His-IleRS
Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorInsertIsoleucyl-tRNA ligase (ileS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Frb-N-TEV
Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-C-TEV 219
Plasmid#100096PurposeSNIPer plasmid, C terminal TEV fused to FKBPDepositorInsertC-TEV
UseRetroviralTagsFKBP and MycExpressionMammalianMutationC terminal domainPromoterCMVAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-ADRβ2 Control Plasmid
Plasmid#101123PurposeExpression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cellsDepositorInsertBeta-2-Adrenergic Receptor (ADRB2 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pORF1-Flag
Plasmid#190565PurposeMammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.DepositorInsertORF1 (from L1.3)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVXT Sox4
Plasmid#101121PurposeSox4 coding regionDepositorInsertSOX4 (Sox4 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Cox8A Control Plasmid
Plasmid#101129PurposeExpression of SNAP-Tagged Cox8A in Mammalian cellsDepositorInsertCox8A (COX8A Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Hyg
Plasmid#158708PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…TagsExpressionBacterialMutationPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG022 (dsr1)
Plasmid#157900PurposeExpresses the dsr1 defense systemDepositorInsertdsr1 (SIR2-DUF4020)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB4697
Plasmid#171125PurposeA plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction.DepositorInsertura4
UseOtherTagsExpressionMutationnon applicablePromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR-Zeo
Plasmid#158709PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…TagsExpressionBacterialMutationPromoterHsp60Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_260d
Plasmid#91126PurposeTransformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV261-Cas12a
Plasmid#158707Purposeexpresses codon-optimized FnCpf1DepositorInsertFnCpf1
UseTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP NFAT5 DBD
Plasmid#14112DepositorInsertNFAT5 (NFAT5 Human)
UseTagsEGFPExpressionMammalianMutationDNA binding domain aa 175 to 471PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Cm
Plasmid#158711PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInserteforCP (for crRNA cloning)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCAG-BoxB
Plasmid#191122PurposeTranscription of 47xCAG and 12xBoxB repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
BoxB
UseSynthetic BiologyTagsExpressionMutationPromotertetracycline-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only