Skip to main content
Addgene
Showing: 621 - 640 of 1174 results

  1. pLenti4/V5-DEST-P85(N564K)

    Plasmid
    #40226
    Depositor
    Lynda Chin
    Insert
    P85(N564K) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Asparagine 564 to Lysine
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  2. pJK655

    Plasmid
    #71632
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to aspartate, phenylalanine-79 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71632-plasmid-map-sequence-id-131652

    Enlarge

  3. pJK689

    Plasmid
    #71642
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine, phenylalanine-79…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71642-plasmid-map-sequence-id-131663

    Enlarge

  4. pJK608

    Plasmid
    #71621
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to aspartate, additional N-term…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71621-plasmid-map-sequence-id-131639

    Enlarge

  5. pJK686

    Plasmid
    #71624
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asn mutant (AaPurE1-S57N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to asparagine, additional N-ter…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71624-plasmid-map-sequence-id-131641

    Enlarge

  6. pJK658

    Plasmid
    #71648
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-40 to asparagine, phenylalanine…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71648-plasmid-map-sequence-id-131644

    Enlarge

  8. pCMVβ-p300.DY-myc

    Plasmid
    #30490
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    aspartic acid 1399 converted to tyrosine (Acetyla…
    Promoter
    Available since
    Oct. 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. pLenti4/V5-DEST-P85(KS459del)

    Plasmid
    #40222
    Depositor
    Lynda Chin
    Insert
    P85(KS459delN) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    amino acids 459-460 replaced with asparagine
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only

  10. pLenti4/V5-DEST-P85(D560Y)

    Plasmid
    #40223
    Depositor
    Lynda Chin
    Insert
    P85(D560Y) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 560 to Tyrosine
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. pJK627

    Plasmid
    #72462
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to aspartate, tyrosine…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72462-plasmid-map-sequence-id-133901

    Enlarge

  12. pJK509

    Plasmid
    #71599
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71599-plasmid-map-sequence-id-131673

    Enlarge

  14. pcDNA3-Casp2-Flag

    Plasmid
    #11811
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 (CASP2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11811-plasmid-map-sequence-id-299764

    Enlarge

  16. pcDNA3.1_RapACR_T111C_N248Q-EYFP

    Plasmid
    #119197
    Purpose
    Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q
    Depositor
    John Spudich
    Insert
    Anion channelrhodopsin RapACR fast mutant T111C_N248Q
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    changed threonine 111 to cysteine and asparagine …
    Promoter
    CMV
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    119197-plasmid-map-sequence-id-230387

    Enlarge

  17. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

    Enlarge

  18. pJK602

    Plasmid
    #71607
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71607-plasmid-map-sequence-id-131684

    Enlarge

  19. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

    Enlarge

  20. pJK510

    Plasmid
    #71600
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-16…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71600-plasmid-map-sequence-id-131674

    Enlarge

Showing: 621 - 640 of 1174 results