Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 13,381 results for: SHI

Showing: 621 - 640 of 13381 results

  1. LentiV_Neo_SIK3_CR_K95M

    Plasmid
    #108106
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108106-plasmid-map-sequence-id-207840

    Enlarge

  4. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

    Enlarge

  7. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid
    #123350
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-Cameleon
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123350-plasmid-map-sequence-id-307103

    Enlarge

  9. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123329
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-AKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123329-plasmid-map-sequence-id-313436

    Enlarge

  13. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48226-plasmid-map-sequence-id-69514

    Enlarge

  18. enIscB

    Plasmid
    #205410
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    205410-plasmid-map-sequence-id-421947

    Enlarge

Showing: 621 - 640 of 13381 results