Addgene

We narrowed to 5,865 results for: pcDNA 3.1

Showing: 621 - 640 of 5865 results

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FLAG-OTUB1 WT-pcDNA3.1

Plasmid

#118209

Purpose

Expresses FLAG-OTUB1 WT in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

Plasmid

#159130

Purpose

expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Use

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-pcDNA3.1-

Plasmid

#52501

Purpose

expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-otmyd88

Plasmid

#217485

Purpose

Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Insert

BFP-P2A-otPKRFL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gβγ-BERKY3

Plasmid

#158219

Purpose

BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSyncytin-1(del480)

Plasmid

#182441

Purpose

hSyncytin-1, deletion 481-538

Depositor

Masaharu Somiya

Insert

ERVW-1 (ERVW-1 Human)

Use

Tags

Expression

Mammalian

Mutation

deletion 481-538

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA

Plasmid

#157750

Purpose

Expression of AURKA targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

Aurora A without the first 30AA + MTS of CoxVIII

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKB-V5/HIS

Plasmid

#205614

Purpose

Expression of human TRKB receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKB receptor tyrosine kinase (NTRK2 Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mCherry

Plasmid

#62632

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Article

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Use

Tags

Flag-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Use

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαq*-BERKY3

Plasmid

#158217

Purpose

BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pY004 (pcDNA3.1-hFnCpf1)

Plasmid

#69976

Purpose

Expresses humanized FnCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hFnCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/-FLI1-C‐(K)‐DYK

Plasmid

#129497

Purpose

Mammalian expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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